转TaLEA基因小黑杨无性系生长性状变异研究  

Variation Analysis of Growth Traits of Transgenic Populus simonii×P. nigra Clones Carrying TaLEA Gene

作　　者：刘殿昆 刘梦然[1] 李志新[1] 汪广宇 李莹[1] 郑密[1] 刘桂丰[1] 赵曦阳[1] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040 [2]黑龙江省大庆市绿化工程有限责任公司,大庆163000

出　　处：《植物研究》2015年第4期540-546,共7页Bulletin of Botanical Research

基　　金：中央高校基本科研业务费专项资金项目(2572014CA16);中国博士后科学基金项目(2014M561315)

摘　　要：以6年生的10个转TaLEA基因和1个非转基因小黑杨无性系为材料,对其生长量、干形、叶片性状、皮孔性状等15个指标进行测定分析。方差分析与遗传参数计算结果表明：11个小黑杨无性系间各性状达极显著差异水平（P〈0.01）,各指标表型变异系数处于1.92%~39.98%,遗传变异系数与表型变异系数接近,重复力处于0.621~0.987,表明基因的转入对无性系各性状的生长有一定的影响,但这种影响主要由遗传因素控制。表型相关分析结果表明转基因小黑杨生长量与干形性状、叶片性状、皮孔性状均呈不同程度的相关性,表明多指标共同作用,控制植株生长发育;利用综合评定法,当入选率为10%,初步选出XL-1和XL-9等2个优良无性系,2个无性系的树高、胸径分别比CK高16.55%和15.38%,比平均值高13.38%和19.46%,遗传增益分别为12.18%和17.42%。本研究为转基因杨树的良种选育提供重要的理论依据。We used 11 Populus simonii× P. nigra clones including ten transgenic poplar clones and one control clone in six-year old to study 15 traits including growth traits, shape of stem, leaf traits, and lenticel traits. By ANOVA analysis, there was significant difference in all the traits among 11 clones（ P 〈 0.01 ）. The coefficients of phenotypic variation （PCV） of different traits ranged in 1.92% -39.98%, the coefficients of genetic variation（GCV） were closed to PCV and repeatability（R） ranged in 0. 621 - 0.987, which indicated that the TaLEA gene transfer can influence the plant growth, but the impact were strong controlled by genetic factor. By phenotypic correlation analysis, there were varying degree correlations between growth traits, stem traits, leaf traits and lenticel traits, which indicated that all the traits combined action on plant growth and development. When the selection rate was 10%, XL-1 and XL-9 were selected as the best clones. The average height and diameter at breast height of these two clones were higher than those of CK（ average of all the clones） by 16. 55% （13.38%） and 15.38% （19.46%）, and the genetic gain of these clones were 12.18% and 17.42% in height and diameter at breast height, respectively.

关 键 词：小黑杨 TaLEA基因 相关分析 遗传增益 

分 类 号：S792.11[农业科学—林木遗传育种]