金属内植物表面微螺纹联合微弧氧化处理对其在骨质疏松条件下生物稳定性的影响  

Effect of surface modification of a metal implant by micro-screw threading plus micro-arc oxidation on its biostability in osteoporotic sheep

作　　者：杨峥[1] 石磊[2] 王陵[3] 郭征[2] 雷伟[2] 张扬[2] 李小康[2] 裴国献[2] 

机构地区：[1]第四军医大学学员旅,西安710032 [2]第四军医大学西京医院骨科 [3]第四军医大学统计教研室

出　　处：《中华创伤骨科杂志》2015年第6期526-531,共6页Chinese Journal of Orthopaedic Trauma

基　　金：国家高技术研究发展计划（863计划）（2007AA032431）

摘　　要：目的 观察经过表面微螺纹复合微弧氧化处理的内植物能否提高其在骨质疏松（OP）绵羊体内生物学表现. 方法 选用6只成年雌性绵羊,建立OP模型.分别于每只绵羊右侧髂骨植入6枚内植物,分为3组,每组2枚.A组：表面未处理组;B组：微螺纹处理组;C组：微螺纹加微弧氧化处理组.术后3个月处死动物,并随机选取3只绵羊在其左侧髂骨分别植入6枚内植物,每组2枚,作为力学实验0点对照.取材标本进行最大推出力实验、Micro-CT检测以及组织学分析. 结果 植入绵羊髂骨3个月后,各组的最大推出力和能量吸收值均较0点提高,差异有统计学意义（P＜0.05）,C组的最大推出力和能量吸收值高于A、B组,差异均有统计学意义（P＜0.05）.Micro-CT检测显示C组的骨体积分数、骨小梁厚度、组织骨密度均优于A、B组,差异有统计学意义（P＜0.05）.组织学显示C组的新骨形成率和骨接触率均优于A、B组,差异有统计学意义（P＜0.05）.结论 表面微螺纹复合微弧氧化处理内植物,有利于提高内植物在OP条件下的生物学表现,增强内植物的稳定性.Objective To observe whether biological performance of a metal implant can be improved after its surface has been modified by micro-screw threading plus micro-arc oxidation in osteoporotic sheep.Methods Osteoporosis was induced in 6 adult female sheep by bilateral ovariectomy and confirmed by dual energy X-ray absorptiometry and test of bone density of lumbar vertebrae after hormone administration and low calcium diet for 12 months.After 6 implants were put into the right ilium of each sheep,they were divided into 3 equal groups：group A （untreated）,group B （treated by micro-screw threading） and group C （treated by micro-screw threading plus micro-arc oxidation）.All the animals were sacrificed at 3 months after implantation.Three of them were randomly chosen as zero point controls,in each of which 6 implants were put into the left ilium and divided into similar 3 treatment groups.Mechanical test,Micro-CT analysis and histological observation were conducted for statistical analysis of the data.Results At 3 months after implantation,the maximal pull-out strength and energy absorption in each experimental group were significantly enhanced compared with each control group （P 〈 0.05）.The maximal pull-out strength and energy absorption in group C were significantly better than in groups A and B （P 〈 0.05）.Micro-CT reconstruction showed that the bone volume fraction,trabecular thickness and bone mineral density in group C were significantly improved compared with groups A and B （P 〈 O.05）.Histological observation showed significantly improved rates of new bone formation and bone contact in group C compared with groups A and B （P 〈 0.05）.Conclusion Biological performance and stability of a metal implant in osteoporotic sheep can be significantly improved after its surface has been modified by micro-screw threading plus micro-arc oxidation.

关 键 词：骨质疏松 骨生成 绵羊 微弧氧化 表面处理 

分 类 号：R318.08[医药卫生—生物医学工程] R687[医药卫生—基础医学]