Bax通道在斑蝥素诱导草地贪夜蛾Sf9细胞凋亡中的作用  

Effects of the Bax channel on cantharidin induced apoptosis of Spodoptera frugiperda Sf9 cells

作　　者：崔高峰[1] 张鹏飞[1] 郝少东[1] 丁凯[1] 王进忠[1] 张志勇[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《应用昆虫学报》2015年第3期641-648,共8页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金项目(30971938;31272099);北京市教委科技创新平台项目(PXM2008_014207_055164);北京市高校人才强教计划项目(PHR-201107135);国家科技支撑计划-果蔬重大病虫害防控技术研究与示范(2012BAD19B06)

摘　　要：【目的】为明确Bax通道在斑蝥素诱导鳞翅目昆虫细胞凋亡过程中的作用。【方法】本文利用Bax通道抑制法测定了斑蝥素诱导鳞翅目昆虫草地贪夜蛾Spodoptera frugiperda(J.E.Smith)细胞系Sf9细胞凋亡过程中线粒体膜电位、线粒体琥珀酸脱氢酶活性及细胞形态等方面的影响。【结果】Bax通道被抑制后,斑蝥素诱导造成的Sf9细胞线粒体膜电位的降低时间延迟,细胞形变率下降,但琥珀酸脱氢酶活性的下降未受影响。【结论】Bax通道参与了斑蝥素引起的Sf9细胞线粒体膜电位改变和细胞形态变化,而与抑制线粒体有关能量代谢的酶无直接关系。[Objectives] To investigate the role of the Bax channel on cantharidin-induced cell apoptosis in lepidopteran insects [Methods] Bax channel blocker was used to determine the roles of mitochondrial membrane potential, succinate dehydrogenase activity in mitochondria, and cell morphology, on apoptosis of Sf9 cells derived from fall the armyworm, Spodoptera frugiperda（J.E. Smith） exposed to cantharidin stress. [Results] The results show that Bax channel blocker can delay the decrease of mitochondrial membrane potential and reduce changes in cell morphology, but fail to stop the decline of succinate dehydrogenase activity, in Sf9 cells after treatment with cantharidin. [Conclusion] Bax related channels play a role in cantharidin-induced changes in mitochondrial membrane potential and destruction of cell morphology. However, the Bax channel does not appear to play a role in the suppression of enzyme activity in mitochondria after cantharidin treatment.

关 键 词：斑蝥素 草地贪夜蛾 Bax通道 细胞形态 琥珀酸脱氢酶 线粒体膜电位 

分 类 号：R285[医药卫生—中药学]