云南省猪戊型肝炎病毒全基因组扩增及进化分析  

Amplification and evolution analysis on full-length genome of swine hepatitis E virus

作　　者：杨臣臣 龙飞燕 毕艳红[1] 李云龙[1] 王珏[1] 禹文海[1] 井申荣[1] 黄芬[1] 

机构地区：[1]昆明理工大学医学院,昆明650500

出　　处：《中国人兽共患病学报》2015年第6期547-551,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助(No.31360619);云南省自然科学基金(No.2013FB032;2011FZ068);中国博士后科学基金(No.2014M562672)资助;杨臣臣和龙飞燕同等贡献~~

摘　　要：目的对猪戊型肝炎病毒全长基因组进行扩增,分析其进化关系,为HEV的感染性克隆研究奠定基础。方法利用巢式逆转录聚合酶链反应(RT-nPCR)和RACE技术对猪粪便中HEV样品进行全基因组扩增,利用DNAStar和MEGA4.0软件进行同源性比较和进化树分析。结果 RT-nPCR方法扩增出HEV的5段目的基因序列和RACE技术扩增出HEV的5′和3′端,序列比对结果正确,HEV全长扩增成功。同源性分析显示其与新疆株(GU119961)同源性较高(96.1%)。系统进化树显示该病毒属于基因4型h亚型猪HEV。结论 HEV全基因组序列扩增成功,为深入研究HEV奠定基础。To analyze the evolution trend and make a foundation for researching the infectious clone of swine Hepatitis E Virus（HEV）,complete genome sequences of swine HEV was amplified by Reverse transcription nested PCR（RT-PCR）.The homology and phylogenetic tree were analyzed by DNAStar and MEGA4.0software,respectively.Results indicated that the full-length genome of swine HEV was successfully amplified by RT-nPCR and RACE.The homology analysis indicated that the swine HEV shared the highest homologies（96.1%）with swine HEV isolated from Xinjiang（GU119961）.The phylogenetic tree suggested that this isolate belonged to swine HEV 4hsub-genotype.

关 键 词：猪戊型肝炎病毒 巢式逆转录PCR 全长基因组分析 

分 类 号：R373.2[医药卫生—病原生物学]