DNA条形码技术在麻蝇亚科鉴定中的应用  

Sarcophaginae identification by DNA-barcoding

作　　者：方义亮[1] 张建庆[1] 肖武[1] 高博[1] 房长天[1] 卞玘璠 徐保海[3] 

机构地区：[1]福建国际旅行卫生保健中心,福州350001 [2]福建医科大学公共卫生学院,福州350001 [3]福建省疾病预防控制中心,福州350001

出　　处：《中国人兽共患病学报》2015年第6期569-573,共5页Chinese Journal of Zoonoses

基　　金：福建检验检疫局科技计划项目基金(No.FK2012-12)~~

摘　　要：目的建立麻蝇亚科种类鉴定的DNA条形码技术,以期解决麻蝇亚科雌性个体无法通过形态鉴定的问题。方法通过对福建口岸截获的黑尾黑麻蝇、黄须亚麻蝇、褐须亚麻蝇、棕尾别麻蝇、酱亚麻蝇等标本进行PCR扩增和序列测定,与BOLD上下载的88条麻蝇亚科蝇种序列进行比对,计算遗传距离、构建系统进化树。结果 5份样本扩增获得COI的长度为658bp,在线BLAST比对结果显示相似度99%以上,遗传距离计算结果显示种内差异0~0.015 4,种间差异0.024 9~0.153 8,系统进化树显示麻蝇亚科同种能聚在一块,Bootstrap值为1 000。结论 DNA条形码技术可以作为麻蝇亚科蝇种鉴定在形态学鉴定之外的重要方法补充。In this study,we aimed to establish method for identifying Sarcophaginae by DNA-barcoding and to solve the problem that Sarcophaginae female species could not be identified by morphology.We amplified and analyzed the sequences of these samples in Fujian frontier such as Helicophagella melanura,Parasarcophaga misera,Parasarcophaga sericea,Boettcherisca peregrine,Parasarcophaga brevicornis,Parasarcophaga dux,Parasarcophaga similis,Parasarcophaga albiceps,Parasarcophaga Pingi,and Seniorwhitea reciproca.Secondly,we aligned these sequences with 88 Sarcophaginae species loaded from BOLD.Thirdly,we estimated the genetic distances and built system evolutionary tree.The result of amplification with 5samples showed that length of the obtained COI sequences were 658 bp.And the result of alignment on BOLD line showed that index of similarity of the same species was above 99%.The result of calculation genetic distances showed that intraspecific difference was 0-0.015 4and interspecific difference was 0.024 9-0.153 8.Phylogenetic tree showed that the same Sarcophaginae species stayed together on value of Bootstrap 1000.It came to a conclusion that DNA-barcoding can be the important method to supplement of morphologically identifying Sarcophaginae.

关 键 词：DNA条形码 COI 蝇类鉴定 

分 类 号：R384.2[医药卫生—医学寄生虫学]