熊胆粉及其替代品对阿糖胞苷致血小板减少模型外周血象及骨髓巨核细胞的影响  

Effect of Bear Bile Powder and Its Succedaneum on Peripheral Blood and Bone Marrow Megakaryocytes in Mouse Model with Thrombocytopenia Induced by Cytosine Arabinoside

作　　者：王青春[1] 娜仁其木格[2] 朱翔慧 王宏宇 白长喜[2] 

机构地区：[1]天津中医药大学,天津300193 [2]内蒙古医科大学蒙医药研究院,呼和浩特010020 [3]内蒙古自治区国际蒙医医院功能检查科,呼和浩特010056

出　　处：《中国实验血液学杂志》2015年第3期779-784,共6页Journal of Experimental Hematology

基　　金：内蒙古自治区自然科学基金(2014MS08124);内蒙古自治区蒙药重点实验室新药创新项目

摘　　要：目的:观察熊胆粉和熊去氧胆酸对血小板减少性紫癜模型小鼠外周血象、骨髓巨核细胞及免疫器官的影响,探讨其作用机制,为研究蒙药熊胆有效替代品提供参考。方法:利用阿糖胞苷诱发血小板减少模型,将70只小鼠随机分为正常、模型、泼尼松、蒙药熊胆(中、高)剂量组和熊去氧胆酸(中、高)剂量7组,并相应进行灌胃治疗。治疗10 d后,对比观察各组外周血象、脾及胸腺脏器指数和骨髓巨核细胞的数量。结果:造模后,模型组血小板(PLT)、白细胞(WBC)及巨核细胞计数明显减少,脾脏脏器指数增大,与正常组比较差异有统计学意义(P<0.05),WBC计数第10天恢复正常;治疗后,各治疗组PLT、WBC及巨核细胞计数明显升高,泼尼松组WBC计数减少,脾脏脏器指数降低,与模型组比较差异有统计学意义(P<0.05),其中蒙药熊胆高剂量组和熊去氧胆酸高剂量组尤为显著。结论:熊胆粉及熊去氧胆酸对阿糖胞苷诱发的血小板减少模型小鼠均有治疗作用,熊去氧胆酸有望代替熊胆作为治疗血小板减少性紫癜的药物。Objective： To investigate the effect of bear bile powder and ursodesxy cholic acid（UDCA） on peripheral blood, bone marrow megakaryocyte and immune organs in mouse model with thrombocytopenia, so as to provide a reference for studying the curative effects of bear bile powder and its succedaneum on thrombocytopenic purpura（TP）. Methods： The mouse model with thrombocytopenia indued by cytosine arabinoside （Ara-C） was established, a total of 70 mice were randomly divided into normal group, model group, prednisone group, bear bile （middle and nigh dose） powder group and UDCA（middle and high dose） group. From the first day of making modal mice in the each group, 0.4 ml/（20 g . d） corresponding drug was administered by infusion. At day 10 after treatment the peripheral blood, spleen and thymus organ index, the number of bone marrow megakaryocyte in each group were compared. Results： compared with the normal group, the Plt, WBC and megakaryocyte counts in model group decreased, the spleen index increased obviously （P 〈 0.05 ）, but the WBC count returned to normal by 10 days; after treatment, compared with model group, the Plt, WBC and megakaryocyte counts of treated groups increased, spleen index decreased significantly （P 〈 0.05 ）, but the WBC count in prednisone group decreased, which in bear bile powder（nigh） group and UDCA （high） group were particularly significant. Conclusion： The bear bile powder and UDCA have been confirmed to have therapeutical effect on thrombocytopenia models induced by Ara-C, UDCA can substitute bear bile powder as a treatment drug for thrombocytopenic purpura.

关 键 词：熊胆粉 熊去氧胆酸 阿糖胞苷 血小板减少性紫癜 外周血象 骨髓巨核细胞 

分 类 号：R558.2[医药卫生—血液循环系统疾病] R973[医药卫生—内科学]