脐带血来源的间充质干细胞对CD34＋细胞增殖的支持作用及其机制(英文)  

Supporting Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells on CD34^+ Cell Proliferation and Its Mechanism

作　　者：徐志娟[1] 盛立霞[1] 楼燕茹[1] 牧启田[1] 张怡[1] 张宜生[1] 欧阳桂芳[1] 

机构地区：[1]宁波大学医学院附属宁波市第一医院,浙江宁波315000

出　　处：《中国实验血液学杂志》2015年第3期802-808,共7页Journal of Experimental Hematology

基　　金：宁波市社会发展科研项目(2010C50019)

摘　　要：目的:探讨脐带血来源的间充质干细胞体外支持造血干细胞扩增的能力及所涉及的可能机制。方法:将来源于脐血的造血干细胞与间充质干细胞共培养14 d,然后计数造血干细胞数与集落形成单位。ELISA方法检测间充质干细胞培养上清中含有的细胞因子。结果:与间充质干细胞共培养的造血干细胞增殖率明显高于单独培养的造血干细胞(P<0.05)。此外,在培养体系中添加外源性细胞因子可明显提高造血干细胞增殖率(P<0.05)。间充质干细胞能分泌多种细胞因子,包括GM-CSF、IL-7、IL-8、IL-11、SCF和SDF-1α。结论:脐带血间充质干细胞作为细胞滋养层可用于体外扩增造血干细胞,间充质干细胞分泌的细胞因子可能介导MSC对HSC增殖的支持作用。Objective：To investigate the ability of UCB-derived MSCs to support the expansion of HSCs ex vivo and the possible mechanisms involved in this process. Methods： HSCs from UCB were co-cultured with UCB-derived MSCs for 14 days, and then the total number of HSCs and colony-forming units （CFU） were detected. Cytokines levels of MSCs supematant were analyzed using ELISA. Results： The proliferation rate of HSCs co-cultured with MSCs was significantly higher than that of cultured HSCs alone （ P 〈 0.05 ）. Furthermore, the addition of exogenous cytokines to the culture system significantly increased the proliferation rate of HSCs （ P 〈 0.05 ）. MSCs had secretion of many cytokines, including GM-CSF, IL-7, IL-8, IL-11, SCF and SDF-1α. Conclusion：UCB-derived MSCs as a feeder layer can be an alternative approach for ex vivo expansion of HSCs, and the cytokines by secreted UCB-MSCs may mediate the supportive role of MSC to HSC proliferation.

关 键 词：脐带血 间充质干细胞 CD34+细胞 造血干细胞 体外扩增 细胞因子 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]