河北省2009～2014年归国人员疟疾实验室检测分析  

Results of laboratory test of malaria infection in overseas returnees from in Hebei province from 2009 to 2014

作　　者：闫冀焕[1] 史玲莉[1] 李云[1] 张志恩[1] 周汝明[2] 

机构地区：[1]河北国际旅行卫生保健中心,河北石家庄050000 [2]河北省人民医院,河北石家庄050000

出　　处：《中国热带医学》2015年第6期743-745,共3页China Tropical Medicine

摘　　要：目的分析河北省2009~2014年归国人员疟疾实验室检测结果,为输入性疟疾的科学防治提供参考依据。方法收集2009~2014年1 284例归国人员血液标本,采用镜检法、疟原虫抗原胶体金法和实时荧光定量PCR三种检测方法核实诊断并进行虫种鉴定。结果 1 284例归国人员,检出疟疾28例（阳性率2.18%）。疟疾病人均为男性,年龄介于20~55岁,中国籍劳务,输入来源地区均为非洲。镜检结果显示：24例为恶性疟,4例为未分型。以疟原虫抗原胶体金法和实时荧光定量PCR法核实鉴定,4例未分型病例中2例为恶性疟,2例为间日疟和恶性疟混合感染。结论河北省2009~2014年归国人员疟疾病例以外出非洲疟疾高发地区劳务人员为主,且多为恶性疟。疟原虫抗原胶体金法和实时荧光定量PCR对于疟疾病例的核实鉴定具有积极的辅助作用。Objective To analyze the results of laboratory test of malaria infection in overseas returnees in Hebei from 2009 to 2014 in Hebei province, and so as to provide the reference for scientific control of imported malaria cases. Methods Totally 1 284 blood samples from returned laborers were examined in Hebei during 2009 to 2014 and malaria cases were detected and the parasite species were identified by light microscopy （LM） , P1asmodium falciparum antigen colloidal gold method and fluorescence quantitative PCR assay. Results Twenty-eight malaria cases were detected from 1 284 laborers returned abroad with the positive rate of 2.18% （28/1 284）and all the patients were male laborers returned from Africa with ages from 20 to 55 years.Microscopy examination revealed that 24 cases were infected with Plasmodium [alciparum and 4 cases were not differentiated. Plasmodium [alciparum antigen colloidal gold method and fluorescence quantitative PCR assay showed that .2 of the 4 undifferentiated cases were identified as Plasmodium falciparum malaria and the other 2 were identified as mixed infection with Plasmodium vivax and Plasmodium falciparum. Conclusion The imported malaria cases in Hebei province from 2009 to 2014 were mainly the laborers returned from hypeendemic areas in Africa and most of them were infected with Plasmodium faleiparum.Plasmodium falciparum antigen colloidal gold method and fluorescence quantitative PCR assay possess assistant role for identification of plasmodium species.

关 键 词：疟疾 实验室检测 河北省 

分 类 号：R181.82[医药卫生—流行病学]