组织芯片检测星形胶质细胞上调基因1与原发性肝细胞癌病理因素的相关性  

Tissue microarray detection for astrocyte elevated gene-1 and its correlation with pathological factors in primary hepatocellular carcinoma

作　　者：李聪[1] 张巍[2] 刘学智[3] 刘博君[1] 张洪海[1] 杨耿侠[1] 生守鹏[1] 孙玉[1] 袁春旺[1] 郑加生[1] 

机构地区：[1]首都医科大学附属北京佑安医院肿瘤微创介入治疗中心,北京市100069 [2]解放军总医院骨科,北京市100853 [3]涞水县医院骨科,河北省保定市074103

出　　处：《中国组织工程研究》2015年第20期3147-3151,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(81472328);佑安肝病艾滋病基金(BJYAH-2011-034);国家科技支撑项目(2012BAI15B08);国家传染病重大专项(2012ZX10002015-002)~~

摘　　要：背景:星形胶质细胞上调基因1是肿瘤发生转移早期的重要标记分子之一,很有可能成为肝癌临床治疗的分子靶标。目的:探索原发性肝细胞癌患者星形胶质细胞上调基因1在其癌组织中的表达水平,并推测其在原发性肝细胞癌患者微创疗效中的地位。方法:采用组织芯片方法和免疫组织化学染色方法检测120例原发性肝细胞癌患者肿瘤组织中星形胶质细胞上调基因1蛋白表达水平,并与其临床病理资料进行相关性分析。结果与结论:原发性肝细胞癌患者星形胶质细胞上调基因1蛋白表达水平与其临床分期和组织分化程度密切相关,且其星形胶质细胞上调基因1蛋白高表达水平与Ki-67阳性率和肿瘤微血管密度有关,但该蛋白高表达与患者的性别及年龄无相关性。说明星形胶质细胞上调基因1蛋白表达水平与常规恶性检测指标相关,检测该蛋白表达水平对于判断原发性肝细胞癌具有重要意义。BACKGROUND：Astrocytes elevated gene-1 is an important molecular marker for early tumor metastasis, which is likely to become the molecular target in the clinical treatment of hepatocelular carcinoma. OBJECTIVE：To investigate the astrocyte elevated gene-1 expression level in the tumor tissues of patients with hepatocelular carcinoma, and to predict its role in the minimaly invasive therapy. METHODS：Tissue microarray and immunohistochemistry methods were applied to detect astrocyte elevated gene-1 protein expression level in the tumor tissues of patients with hepatocelular carcinoma, and their clinical-pathological data were colected for correlation analysis with the astrocyte elevated gene-1 protein expression level. RESULTS AND CONCLUSION：The protein expression level of astrocyte elevated gene-1 was closely related to clinical stage and tissue differentiation degree in patients with hepatocelular carcinoma. In addition, the high expression of astrocyte elevated gene-1 protein was correlated with the positive rate of Ki-67 and tumor micro-vessel density. However, the protein expression had no correlation with gender and age in these patients. Taken together, the protein expression level of astrocyte elevated gene-1 is correlated with conventional malignant indexes, which is of great significance for the judgment of hepatocelular carcinoma.Subject headings： Carcinoma, Hepatocelular; Microchip Analytical Procedures; Ki-67 Antigen; Carcinoma Funding： the National Natural Science Foundation of China, No. 81472328; YouAN Fund for Liver Diseases and AIDS, No. BJYAH-2011-034; the National Science and Technology Support Program, No. 2012BAI15B08; the National Special Fund for Infectious Diseases, No. 2012ZX10002015-002

关 键 词：组织构建 组织工程 星形胶质细胞上调基因1 组织芯片 肝细胞癌 免疫组化 Ki-67 临床病理 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]