IL-31和他克莫司对Hacat细胞NT4表达的影响  

Effects of IL-31 or tacrolimus on expression of NT4 in human Hacat cells

作　　者：李晓静[1] 王华[1] 

机构地区：[1]重庆医科大学附属儿童医院皮肤科、儿童发育疾病研究教育部重点实验室、儿科学重庆市重点实验室、重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014

出　　处：《重庆医科大学学报》2015年第4期612-615,共4页Journal of Chongqing Medical University

基　　金：2010年中华医学会皮肤病分会-安斯泰来皮肤病学研究基金资助项目

摘　　要：目的:探讨白介素-31(interleukin-31,IL-31)、他克莫司对角质形成细胞神经营养素-4(neurotrophin-4,NT4)表达的影响。方法:培养人角质形成细胞株(Hacat细胞),采用不同浓度他克莫司作用24 h,噻唑蓝比色法(MTT法)检测细胞增殖活性。IL-31、他克莫司单独或共同作用于Hacat细胞24 h后,采用real-time PCR法检测NT4 m RNA表达,酶联免疫吸附法(ELISA)分别检测12、24 h各组细胞上清液中NT4含量。结果:低浓度他克莫司(10、100)ng/ml对细胞活性无明显影响(P>0.05),高浓度(1 000 ng/ml)时细胞活性受到抑制(P=0.002)。IL-31作用Hacat细胞后,其NT4m RNA表达和上清中NT4含量均增加(P<0.05);100 ng/ml他克莫司作用24 h后他克莫司组和联合作用组细胞NT4 m RNA表达量和细胞上清中NT4含量均分别低于对照组和IL-31组(P<0.05)。结论:IL-31可促进角质形成细胞NT4表达,他克莫司可能通过降低角质形成细胞NT4表达水平而发挥其抗瘙痒作用。Objective：To investigate the effects of interleukin-31（IL-31）or tacrolimus on expression of neurotrophin-4（NT4） in human Hacat cells. Methods ：Human keratinocyte cell line（Hacat cells）was cultured and treated with different concentrations of tacrolimus and cell proliferation was determined by the MTT assay. Cultured Ha Ca T cells were treated with IL-31 alone or in combination with tacrolimus for 24 h. Real-time PCR was used to detect the m RNA of NT4. Levels of NT4 in the culture supernant were measured with ELISA. Results：Tacrolimus with low concentration（10,100） ng/ml had mild effect on cell proliferation（P 0.05）,while that with high concentration（1 000 ng/ml） inhibited cell proliferation（P =0.002）. Levels of NT4 in the culture supernant and NT4 m RNA expression were significantly increased in the presence of IL-31. Levels of NT4 in the culture supernant and NT4 m RNA expression were significantly decreased with IL-31 and 100 ng/ml tacrolimus acting alone or in combination for 24 h. Conclusion：The expression of NT4 of keratinocyte is up-regulated by IL-31. Tacrolimus might achieve its anti-pruritus effect in atopic dermatitis by decreasing the NT4 expression of keratinocytes.

关 键 词：白介素-31 他克莫司 神经营养素-4 特应性皮炎 

分 类 号：R758.23[医药卫生—皮肤病学与性病学]