玻璃化冷冻胚胎与新鲜胚胎移植对子代小鼠生长发育的影响  

Influence of vitrified versus fresh embryo transplantation on the growth and development of mouse offspring

作　　者：周经委 吴蓉花[2] 郭里[2] 孙琴[2] 丁晓萌 随刘才[2] 陈莉[2] 

机构地区：[1]江苏大学临床医学院,镇江医学硕士研究生212013 [2]南京军区南京总医院生殖医学中心,南京210002

出　　处：《医学研究生学报》2015年第6期569-573,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(31371520)

摘　　要：目的辅助生殖技术会引起子代缺陷,文中通过比较经体外受精（invitro fertilization,IVF）的新鲜胚胎与玻璃化冷冻胚胎移植后所产生的子代小鼠生长状况,以探讨胚胎冷冻对IVF后代生长发育的影响。方法实验共分为3组,对照组（12只）：小鼠自然交配所产生子代;鲜胚移植组（9只）：收集IVF后的小鼠囊胚,直接将囊胚进行移植;冻胚移植组（9只）：收集IVF后的小鼠囊胚,进行玻璃化冷冻胚胎,复苏后移植到假孕母鼠子宫内,分析比较3组子代小鼠体重的变化,性成熟期体脂。结果鲜胚移植组与冻胚移植组胚胎在高倍显微镜下形态无明显差异。冻胚移植组0～12周子代小鼠体重高于对照组（P〈0.01）,鲜胚移植组子代小鼠0周、2～6周体重高于对照组（P〈0.05）;冻胚移植组性成熟期脂肪含量[（9.32±4.34）g]高于鲜胚组[（5.24±2.56）g]和对照组[（2.80±0.48）g],差异有统计学意义（P〈0.05）。冻胚移植组肌肉含量[（33.77±4.22）g]高于对照组[（27.50±1.79）g],差异有统计学意义（P〈0.01）。结论鲜胚移植技术对子代的生长状况干扰小于玻璃化冷冻技术。Objective Assisted reproductive technology（ ART） is associated with an increased incidence of offspring defects. The aim of this study was to investigate the impact of embryo vitrification in in vitro fertilization（ IVF） on the growth and development of the offspring by comparing the development status of mouse offspring from fresh embryo versus vitrified embryo transplantation. Methods This study included three groups of mouse offspring,from natural fertilization（ n = 12）,fresh embryo transplantation（ FET,n = 9）,and vitrified embryo transplantation（ VET,n = 9）. We compared the body weight and body fat content in the sexually mature stage among the three groups of mice. Results No obvious differences were found in the morphology of the embryos between the FET and VET groups. The body weight of the mouse offspring was significantly higher in the VET than in the natural control group at 0 postnatal（ PN） week（ P〈0. 01） and 12 PN weeks（ P〈0. 01）,and so was it in the FET group than in the control at 0 PN week（ P〈0. 01） and 6 PN weeks（ P〈0. 05）. The body fat content at sexual maturity was remarkably higher in the VET than in the FET and control groups（ [9. 32 ± 4. 34] vs [5. 24 ± 2. 56] and [2. 80 ±0. 48] g,P〈0. 05）,and so was the muscle content in the VET than in the control（ [33. 77 ± 4. 22]vs [27. 50 ± 1. 79]g,P〈0. 01）.Conclusion In IVF,fresh embryo transplantation has less interference with the growth and development of mouse offspring than vitrified embryo transplantation.

关 键 词：体外受精 新鲜胚胎 玻璃化冷冻胚胎 子代 小鼠 生长发育 

分 类 号：R714[医药卫生—妇产科学]