香卿止痛喷雾剂镇痛抗炎作用的药效学研究  

Study on the anti-inflammatory and analgesic effects of xiangqingzhitong spray

作　　者：武涛 刘文雅[2] 王争[2] 王曙东[2] 

机构地区：[1]南京军区南京药品器材供应站,南京药学硕士210003 [2]南京军区南京总医院制剂科,南京210002

出　　处：《医学研究生学报》2015年第6期637-641,共5页Journal of Medical Postgraduates

基　　金：南京军区医学科技创新基金(10MA102);南京军区医药卫生科研基金(ZX26)

摘　　要：目的香卿止痛喷雾剂(简称XQ喷雾剂)是按中药五类研究的新药,为阐明药物的功能主治,文中研究了XQ喷雾剂的镇痛、抗炎作用,为临床应用提供试验依据。方法选择雄性SPF级健康大鼠(60只)和SPF级健康小鼠(雄性120只,雌性60只),随机挑选小鼠60只(雌雄各半)分为空白A组、云南白药A组、香卿喷雾剂低、中、高A组(n=10),通过小鼠电刺激致痛试验,观察喷雾剂对电刺激致痛痛阈值的影响;另随机挑选小鼠60只(雌雄各半)分为空白B组、云南白药B组、香卿喷雾剂低、中、高B组(n=10),通过小鼠尾压痛试验,观察喷雾剂对小鼠尾压痛痛阈值的影响,研究该药的镇痛作用。另将60只雄性小鼠分为空白C组、云南白药C组、香卿喷雾剂低、中、高C组(n=10),采用二甲苯致小鼠耳廓肿胀模型,观察该药的抗炎作用;将60只大鼠分为空白D组、云南白药D组、香卿喷雾剂低、中、高D组(n=10),采用角叉菜胶致大鼠足跖肿胀模型,观察喷雾剂的抗炎作用。结果小鼠电刺激致痛试验表明XQ喷雾剂中、高剂量A组能显著提高小鼠电刺激痛阈值,给药后15 min和30 min镇痛百分率达90%,与空白对照比差异有统计学意义(P<0.05),与云南白药A组比较,差异无统计学意义(P>0.05);XQ喷雾剂高剂量B组于给药后15、30 min均能极显著提高小鼠尾压痛阈值,与空白B组比差异有统计学意义(P<0.01),与云南白药B组比较,XQ喷雾剂高剂量B组对小鼠尾压痛阈值差异无统计学意义(P>0.05)。并且XQ喷雾剂中剂量C组能显著减轻二甲苯所致小鼠耳廓肿胀,与空白C组比差异有统计学意义[(4.41±4.55)mg vs(9.15±3.94)mg,P<0.05],高剂量C组能极显著减轻二甲苯所致小鼠耳廓肿胀,与空白C组比差异有统计学意义[(3.62±3.56)mg vs(9.15±3.94)mg,P<0.01];XQ高剂量D组于给药后30 min对角叉菜胶所致大鼠足跖肿胀有显著的抑制作用,与空白D组比差异有统计学意义[(1.19±0.94)vs(2.54±1.28)Objective Xiangqingzhitong spray（ XQ spray） is a kind of class V new Chinese drugs. This paper studied the anti-inflammatory and analgesic effects of XQ spray to provide experiment evidence for clinical application. Methods Mice were divided into blank control A group,yunnan baiyao aerosol A group,voltaren A group and low,middle and high dose A group of XQ spray. Analgesic effects were studied by observing effect of XQ spray for pain threshold in electrical stimulation test. Mice were divided into blank control B group,yunnan baiyao aerosol B group,voltaren B group and low,middle and high dose B group of XQ spray. Analgesic effects were studied by observing the effects of XQ spray for pain threshold in tail pain tenderness test. Mice were divided into blank control C group,yunnan baiyao aerosol C group,voltaren C group and low,middle and high dose C group of XQ spray. Anti-inflammatory effects were observed by the models of ear swelling induced by xylene in mice. Rats were divided into blank control D group,yunnan baiyao aerosol D group,voltaren D group and low,middle and high dose D group of XQ spray. Anti-inflammatory effects were observed by the models of paw edema induced by carrageenin in rats.Results Electrical stimulation test showed that middle and high dose A group of XQ spray could enhance the pain threshold and the analgesia percentage reached 90% after the administration of 15 min and 30 min. Compared with control group,significant difference was found in medium and high dose A group（ P〈0. 05）. High dose B group of XQ spray could enhance pain threshold in tail pain tenderness test markedly after the administration of 15 min and 30 min and the difference was significant compared with control group（ P〈0. 01）. Middle dose C group could inhibit the ear swelling induced by xylene in mice and the difference was significant compared with control group（ [4. 41 ± 4. 55]mg vs [9. 15 ± 3. 94]mg,P〈0. 05）. High dose C group could inhibit the ear swelling induced by xylene in mice and signif

关 键 词：XQ喷雾剂 镇痛作用 抗炎作用 

分 类 号：R28[医药卫生—中药学]