卵巢移植对去势大鼠海马神经元和雌激素膜受体GPR30表达的影响  

The effect of ovarian transplantation on hippocampal neurons and GPR30 expressions in ovariectomized rat

作　　者：刘芳[1,2,3] 黑常春[1,2] 孔斌[1,2] 常青[1,2] 郑小敏[1,2] 吴凯[1,2] 周文献[1,2] 朱万平[1,2] 赵承军[1,2,4] 

机构地区：[1]宁夏医科大学人体解剖与组织胚胎学系 [2]宁夏医科大学生育力保持教育部重点实验室宁夏生殖与遗传重点实验室,宁夏银川750004 [3]陕西中医学院组织胚胎学教研室,陕西西安712046 [4]宁夏医科大学医学科学技术研究中心,宁夏银川750004

出　　处：《基础医学与临床》2015年第7期889-893,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81160084);宁夏自然科学基金(NZ11108)

摘　　要：目的观察去势大鼠卵巢移植后海马神经元形态、尼氏体数量和雌激素膜受体GPR30表达的变化,评价卵巢移植对海马神经元功能的保护作用。方法成年雌性SD大鼠分为对照组、去势组和卵巢移植组。对照组行假手术,去势组行双侧卵巢切除,卵巢移植组在切除双侧卵巢7 d后,肾被膜下移植出生3 d内乳鼠的卵巢。于移植后的7、14、21和28 d,尼氏染色观察海马神经元形态、尼氏体数量;免疫组织化学法和Western blot检测GPR30蛋白的表达。结果去势组随去势时间延长,海马神经元排列散乱,胞体皱缩,核固缩深染,神经元尼氏体数量减少(P<0.05),GPR30表达减少(P<0.05)。卵巢移植组随移植时间的延长,细胞形态逐渐好转并与正常形态接近,海马神经元内尼氏体数量和GPR30蛋白表达逐步恢复,并接近对照组。结论卵巢移植可以逆转去势所致神经元形态改变,并提高GPR30蛋白的表达,促进海马神经元蛋白合成功能的恢复。Objective To observe the change of hippocampal neurons,Nissl body and GPR30 expression on castrated rats after ovarian transplantation to evaluate the protective effect of ovarian transplantation on hippocampal neurons.Methods The adult SD rats were divided into control group （sham operation）,castration group （excision of two ovaries） and transplantation group （transplantation of ovary of 3 days neonate rat under the renal capsule after castration）.7,14,21 and 28 d after the ovarian transplantation,the brains were removed for evaluating the morphologic change of hippocampal neurons,amount of Nissl bodies and the expression of GPR30 by Nissl staining,immunohistochemistry and Western blot in three groups.Results Normal hippocampus and plentiful Nissl bodies were seen in control group,the GPR30 was expressed in cytoplasm of hippocampus neurons.Compared with the control group,the layers of hippocampus neurons were reduced and the shrinkage of cytoplasm and nuclei were seen in hippocampus neurons.The amount of Nissl bodies and expression of GPR30 were decreased time-dependently in the castration group,and increased time-dependently in the transplantation group with recovery of neuron structures.Conclusions The ovarian transplantation can reverse the morphological changes of castration-induced hippocampus neurons and improve the expression of GPR30 protein,and promote the recovery of protein synthesis function in hippocampal neurons.

关 键 词：卵巢去势 卵巢移植 海马神经元 GPR30 

分 类 号：R339.22[医药卫生—人体生理学]