肠源性TPH1、LRP5表达及血清5-HT水平与雄性大鼠增龄性骨量丢失相关  

Relationships between enterogenous TPH1,expression of LRP5,serum level of 5-HT and aging bone mass loss of the male rats

作　　者：胡庭毅 张健[1] 陈潇[1] 蔡枭[1] 熊科[1] 

机构地区：[1]重庆医科大学第一附属医院骨外科,重庆404100

出　　处：《基础医学与临床》2015年第7期934-937,共4页Basic and Clinical Medicine

基　　金：重庆市渝中区科委基金(20110405)

摘　　要：目的观察不同月龄SD雄性大鼠十二指肠TPH1、LRP5表达水平及血清5-HT水平,分析其在增龄中的变化及增龄性骨量丢失的相关性。方法分别选取2、6、12、15和18月龄大鼠各6只,共30只,按照月龄分组以模拟增龄性模型。分别用RT-PCR法、Western blot法检测十二指肠TPH1和LRP5的mRNA和蛋白表达,用ELISA方法检测血清5-HT水平,双能骨密度仪测定大鼠离体股骨骨密度。结果十二指肠TPH1 mRNA和蛋白表达及血清5-HT水平随年龄的增长而增加,与增龄性骨量丢失呈负相关性(P<0.001)。结论肠源性5-HT及其关键合成限速酶TPH1表达在增龄性骨量丢失中发挥着重要作用,可能成为老年性骨质疏松潜在治疗干预靶。Objective To observe the expression levels of duodenal TPH1 and LRP5 as well as the level of serum 5-HT in SD male rats of different months,and analyze their changes with the increase of age and the correlation with aging bone mass loss.Methods Six male SD rats at the age of 2,6,12,15 and 18 months respectively （30in total） were selected in this study for each group and they were grouped according to their ages so as to simulate the physiological aging model.RT-PCR and Western blot methods were employed respectively in this study to detect the mRNA and the expression of protein level of duodenal TPH1,LRP5 in rats of different months.Moreover,the ELISA method was used to check the level of serum 5-HT and the Dual-energy bone mineral density method was used to determine the bone mineral density of rats＇ femur in vitro.Results Both the expression level of the duodenal TPHI mRNA and protein as well as the level of serum 5-HT increased with the growth of the age,but they showed the negative correlation with the aging bone mass loss （P ＜ 0.001）.Conclusions The enterogenous 5-HT and the expression of TPH1,which is the key enzymes for the speed limit,play an important role in the aging bone mass loss,and they might become the new potential treatment andintervention targets for senile osteoporosis.

关 键 词：LRP5 TPH1 5-HT 老年性骨质疏松 

分 类 号：R580[医药卫生—内分泌]