旋覆代赭汤不同煎煮工艺对胃肠道影响的比较研究  

Comparative Study on Gastrointestinal Tract of Different Decoction Process of Xuanfu-Daizhe

作　　者：吕兴[1,2] 许惠琴[1,2] 吕高虹[1] 陈玉萍[1,2] 吴云皓[1,2] 秦昆明[1,3] 戴国英[1,2] 沈红胜 孙晓晓[1,2] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药药效与安全性评价重点实验室,江苏南京210023 [3]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023

出　　处：《南京中医药大学学报》2015年第4期337-340,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：中医药行业科研专项项目(201007010)

摘　　要：目的 通过考察旋覆代赭汤对小鼠胃排空、小肠推进和离体肠段张力的影响,比较不同煎煮工艺在作用上的一致性和差异性。方法 分别采用正常小鼠和阿托品造模小鼠,用营养性半固体糊加活性炭法观察旋覆代赭汤传统煎（CJ）、机器常压煎（JCJ）、机器高压煎（JGJ）3种煎煮方法对小鼠胃内容物残留率和小肠推进率的影响;分别采用正常大鼠和阿托品所致大鼠肠段张力实验,考察CJ、JCJ、JGJ 3种煎煮方法对大鼠十二指肠段张力的作用。结果 旋覆代赭汤3种煎煮方法对正常小鼠胃内残留率作用不明显,而CJ小剂量、JCJ及JGJ 2个剂量组均能显著增强正常小肠推进率（P〈0.05～0.01）,尤以JCJ大剂量组更为明显;但在阿托品抑制作用的基础上,CJ小剂量、JCJ和JGJ大剂量组能显著减少胃内残留率（P〈0.05～0.01）,CJ、JCJ和JGJ各剂量组显著增强小肠推进率（P〈0.05～0.01）,尤以CJ各剂量组和JGJ大剂量组更为明显;3种煎煮方法均能提高大鼠肠段张力增强率,在正常情况下以JGJ组更为显著（P〈0.01）,在受阿托品抑制作用下,JCJ和JGJ组与更为显著（P〈0.05～0.01）,但3种煎煮方法比较未见显著差异（P〉0.05）。结论 旋覆代赭汤具有协调胃肠功能的作用,JCJ和JGJ的作用相近或略优于CJ法,为目前临床代煎采用的JCJ法和JGJ法提供实验依据。OBJECTIVE To compare the similarities and differences of different decoction process on gastrotestinal tract, by observing the effects of Xuanfu-Daizhe decoction on gastric emptying, small intestinal propulsion and in vitro intestinal seg- ment tension in mice. METHODS The normal and atropine-model mice were given nutritious semisolid paste plus charcoal, respectively, to observe the effects on stomach residual rates and small intestine advance rates of traditional decoction （CJ）, at- mospheric-pressure machine decoction （JCJ） and high-pressure machine decoction （JGJ）. The intestinal segment tension exper- iment were performed in both normal and atropine-induced rats, to observe the effect on duodenal tension of three decoction methods （CJ, JCJ and JGJ）. RESULTS The effects of three decoction process methods of Xuanfu-Daizhe on stomach residual rates in normal mice were inconspicuous. CJ group of low dose, JCJ and JGJ groups of both low and high doses groups dis- played significantly increased small intestine advance rates （P〈0.05～0.01）. In model mice, the CJ group of low dose, JCJ and JGJ groups of high doses reduced stomach residual rates significantly （P〈0.05～0.01）. The small intestine advance rates were increased obviously in all decoction process method groups （P〈0.05～0.01）, especially in the CJ groups and JGJ group of high dose （P〈0.01）. All three decoction methods improved intestinal segment tension enhancement rates. It was more sig- nificant （P〈0.01） in JGJ group in normal rats, while more obvious （P〈0.05～0.01） in JCJ and JGJ groups in atropine-in-duced rats. No obvious differences were observed among three decoction process methods. CONCLUSION Xuanfu-Daizhe de- coction can coordinate gastrointestinal function. The decoction process methods of JCJ and JGJ is equivalent to or better than CJ method, which provides experimental basis for current clinical application of JGJ and JCJ decoction methods.

关 键 词：旋覆代赭汤 胃排空 小肠推进 肠段张力 

分 类 号：R285.5[医药卫生—中药学]