针刺对ApoE^(-/-)小鼠肝脏病理变化因素的影响  

Effect of Acupuncture on Liver Pathological Changes of ApoE^(-/-) Mice

作　　者：艾炳蔚 陶文剑[2,3] 詹冬梅[4] 

机构地区：[1]南京中医药大学附属医院,江苏南京210023 [2]南京医科大学第一附属医院,江苏南京210029 [3]南京中医药大学第二临床医学院,江苏南京210023 [4]盐城市第三人民医院康复科,江苏盐城224005

出　　处：《南京中医药大学学报》2015年第4期345-348,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省人事厅卫生行业2011年"六大人才高峰"资助项目

摘　　要：目的观察针刺干预ApoE-/-小鼠后肝脏细胞的形态和肝脏中低密度脂蛋白受体(LDLR)蛋白的表达变化以及各组小鼠间血浆氧化低密度脂蛋白(Ox-LDL)含量的差异,探讨针刺对ApoE-/-小鼠肝脏病理因素的影响以及对动脉粥样硬化的改善机制。方法 6周龄ApoE-/-小鼠喂食高脂饲料至20周龄,判断动脉粥样硬化模型造模成功后,将其随机分为药物对照组(F组)、针灸治疗组(A组)、模型对照组(M组),每组10只,继续给予高脂高胆固醇饮食喂养。同时,将5只喂食普通饲料的同龄C57BL/6J小鼠作为空白对照组(BC组)。F组给予辛伐他汀灌胃治疗,40mg/(kg·d),A组给予针刺内关、后三里(足三里)、三阴交、神门治疗。治疗11周后,采集各组小鼠血浆行ELISA检测各组小鼠血浆Ox-LDL含量;取小鼠肝脏,行HE染色观察各组小鼠肝脏细胞的形态变化,行免疫组化和Western blot法检测各组小鼠肝脏LDLR蛋白表达的差异。结果针刺后肝脏内细胞LDLR蛋白表达显著增高(P<0.05),肝脏细胞内脂滴变小,肝脏炎症减轻,血浆Ox-LDL含量显著降低(P<0.05)。结论针刺治疗可以调节肝脏内脂质代谢,达到活血化瘀的疗效,从而抑制动脉粥样硬化发展的目的。OBJECTIVE To observe the morphology change of liver ceils, expression of low density lipoprotein receptor （LDLR） in liver cells and content differences of oxidized low density lipoprotein （Ox-LDL） between mice groups. To explore the effect of acupuncture on liver pathological changes of ApoE-/- mice and the mechanism of atherosclerosis improvement. METHODS 34 ApoE / male mice of 6 weeks were fed high forage until 20 weeks. 4 of them were selected randomly and eu- thanized to observe morphology change of aorta sinus plaque with HE staining. After the model were successfully built, other 30 mice were divided randomly into drug control （F） group, acupuncture （A） group and model control （M） group, with 10 in each group, continuing high fat and cholesterol diet. Meanwhile, 5 C57BL/6J male mice were fed ordinary forage as blank con- trol （BC） group. F group was administered with simvastatin of 40 mg/（kg, d） by oral gavage. A group was treated by low-fre- quency electro-acupuncture at neiguan, shenmen, housanli and sanyinjiao points. After 11 weeks treatment, blood specimen of all mice were collected to detect the contents of Ox-LDLwith ELISA. Mice livers were isolated to observe the morphology change of liver cells with HE staining. Immunohistochemical staining and Western blot were applied to detect the expression of LDLR in liver cells of each group. RESULTS Acupuncture enhanced the expression of LDLR in liver（P〈0.05）, diminished the lipid globules in liver cells, improved the inflammatory state and reduced the Ox-LDLcontent significantly （P〈0.05）. CONCLUSION Acupuncture treatment can promote blood circulation and remove blood stasis by regulating lipid metabolism in the liver, and prevent the development of atherosclerosis.

关 键 词：氧化低密度脂蛋白 低密度脂蛋白受体 针刺 动脉粥样硬化 

分 类 号：R245.31[医药卫生—针灸推拿学]