糖网明目颗粒提取物对缺氧/高糖状态下血管内皮细胞肿瘤坏死因子-α、白细胞介素-1α表达的影响  

Effects of Tangwang Mingmu Granules on Expressions of TNF-α and IL-1α in Vascular Endothelial Cellsunder Hypoxia/High Glucose

作　　者：蒿长英[1] 陈明霞[2] 郭平 马文斌[1] 吕海波 杜佩佩[1] 甄林娜 刘晔 连增林 

机构地区：[1]汉典集团北京红太阳药业有限公司,北京100103 [2]北京中医药大学,北京100029 [3]汉典集团北京汉典制药有限公司,北京100103 [4]汉典集团北京汉典中医研究院,北京100103

出　　处：《中国中医药信息杂志》2015年第8期50-54,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市科技计划"十病十药"中药专项(Z121102001112007)

摘　　要：目的观察糖网明目颗粒提取物对缺氧/高糖状态下血管内皮细胞EA.hy926炎症相关因子的影响,并探讨其作用机制。方法以Co Cl2和高浓度葡萄糖干预人脐静脉内皮细胞EA.hy926复制缺氧和高糖模型,MTT法检测细胞增殖能力,半定量RT-PCR检测炎症相关基因表达,ELISA检测炎症相关蛋白表达。结果缺氧和高糖均能促进EA.hy926细胞增殖,显著上调肿瘤坏死因子-α(TNF-α)、白细胞介素-1α(IL-1α)基因和蛋白表达(P<0.05)。药物干预后,细胞增殖能力降低,TNF-α、IL-1α基因和蛋白表达水平显著降低(P<0.05)。结论糖网明目颗粒提取物可能通过调控缺氧/高糖状态下内皮细胞TNF-α、IL-1α等炎症相关因子的m RNA和蛋白表达从而达到防治糖尿病视网膜病变的目的。Objective To observe the effects of Tangwang Mingmu Granules on inflammationassociatedcytokines in vascular endothelial cells EA.hy926 under hypoxic/high glucose.Methods CoCl2 and high glucose were used to make the hypoxic and high glucose model in humanumbilical vein endothelial cell line EA.hy926. EA.hy926 cell proliferation was measured by MTTmethod. Gene and protein expression levels of inflammatory factors were detected bysemi-quantitative RT-PCR and ELISA assay. Results Both hypoxia and high glucose promotedEA.hy926 cell proliferation. The gene and protein expressions of TNF-α and IL-1α weresignificantly up-regulated under the same condition （P 〈0.05）. The cell proliferation, TNF-α, IL-1αmRNA and protein expression levels were significantly reduced by treatment of Tangwang MingmuGranules （P 〈 0.05）. Conclusion The preventive and therapeutic effect of Tangwang MingmuGranules on diabetic retinopathy might be related to down-regulate the expression ofinflammation-associated cytokines of mRNA and protein, such as TNF-α and IL-1α.

关 键 词：糖网明目颗粒提取物 糖尿病视网膜病变 肿瘤坏死因子-α 白细胞介素-1Α 人脐静脉内皮细胞 

分 类 号：R285.5[医药卫生—中药学]