HPLC测定气血双补口服液中芍药苷与淫羊藿苷含量  

Content Determination of Paeoniflorin and Icariin in Qixue Shuangbu Oral Liquid by HPLC

作　　者：任海祥[1] 乔立业[1] 黄鲁[1] 贾佳[1] 陆崟[1] 苏华[1] 

机构地区：[1]南京军区南京总医院制剂科,江苏南京210002

出　　处：《中国中医药信息杂志》2015年第8期80-82,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：军队医疗机构制剂标准提高科研专项课题面上项目(13ZJZ15-2)

摘　　要：目的 改进气血双补口服液的质量标准,为制剂质量提供保障.方法 采用高效液相色谱法同时测定制剂中芍药苷和淫羊藿苷的含量.色谱柱为Agiltnt TC-C18（2）柱（250 mm×4.6 mm,5 μm）,流动相为乙腈-0.1%磷酸梯度洗脱,流速为1.0 mL/min,柱温为25 ℃,检测波长为232 nm.结果 芍药苷、淫羊藿苷分别在0.542～8.677 μg（r =0.999 9）、0.185～2.963 μg（r =0.999 9）范围内有良好的线性关系,平均回收率分别为99.29%、98.91%,RSD 分别为1.06%、1.97%（n =6）.结论 该方法准确易行,便于该制剂的质量控制.Objective To improve the quality standard of Qixue Shuangbu Oral Liquid;To provideguarantee for the quality of oral liquids. Methods HPLC was used to simultaneously determine thecontents of paeoniflorin and icariin in the oral liquid. The determination was performed on aAgiltnt TC-C18 （2） column （250 mm × 4.6 mm, 5 μm） with the mobile phase of acetonitrile and 0.1%H3PO4 gradient mode. The flow rate was 1.0 mL/min;the column temperature was set at 25 ℃;the detection wavelength was 232 nm. Results The linear ranges of paeoniflorin and icariin wereobtained between 0.542-8.677 μg （r =0.999 9） and 0.185-2.963 μg （r =0.999 9）, with the averagerecoveries （n =6） of 99.29% （RSD=1.06%） and 98.91% （RSD=1.97%）, respectively. Conclusion Thismethod is accurate and feasible, which can be used conveniently in quality control of QixueShuangbu Oral Liquid.

关 键 词：气血双补口服液 芍药苷 淫羊藿苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]