抗痨清肺颗粒HPLC特征图谱及液质联用分析  

HPLC Characteristic Chromatogram and HPLC-MS/MS Analysis of Kanglao Qingfei Granules

作　　者：唐林[1] 罗纯清 夏新华[1] 曾建国[2] 刘艳科[2] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]长沙市中心医院,湖南长沙410004

出　　处：《中国中医药信息杂志》2015年第8期88-91,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技重大专项-重大新药创制(2013ZX10005004003-001)

摘　　要：目的 建立抗痨清肺颗粒高效液相色谱（HPLC）特征图谱.方法 采用Kromasil C18色谱柱（4.6 mm×250 mm,5 μm）,以乙腈-1%醋酸水溶液为流动相进行梯度洗脱（0～50 min,5%→15%乙腈;50～70 min,15%→25%乙腈;70～80 min,25%→40%乙腈;80～90 min,40%→65%乙腈;90～120 min,65%→95%乙腈）,流速1.0 mL/min,检测波长290 nm,柱温30 ℃;采用液质联用（HPLC-MS/MS）技术进行色谱峰组分的鉴定.结果 10 批样品相似度均〉0.995,确立了13 个共有特征峰,对其中10 个共有峰进行了来源归属,对其中8 个共有峰进行了成分鉴别.结论 建立的HPLC 特征图谱可用于抗痨清肺颗粒的质量控制.Objective To establish HPLC characteristic chromatogram of Kanglao QingfeiGranules. Methods HPLC analysis of samples was performed on Kromasil C18 column （4.6 mm ×250 mm, 5 μm）, with acetonitrile-1% glacial acetic acid as the mobile phase of gradient elution（0-50 min, 5%→15% acetonitrile;50-70 min, 15%→25% acetonitrile;70-80 min, 25%→40%acetonitrile;80-90 min, 40%→65% acetonitrile, 90-120 min, 65%→95% acetonitrile）;the volumeflow rate was 1.0 mL/min;detection wavelength was set at 290 nm;column temperature was30 ℃. Chromatographic peaks were identified by HPLC-MS/MS method. Results The similaritydegrees of 10 batches of samples were all greater than 0.995, and 13 chromatographic peaks weredetermined as common characteristic peaks, of which 10 peaks were confirmed in the sourceattribution and 8 peaks were identified in chemical component. Conclusion The established HPLCcharacteristic chromatogram can be used for the quality control of Kanglao Qingfei Granules.

关 键 词：抗痨清肺颗粒 特征图谱 高效液相色谱法 液质联用 

分 类 号：R284.1[医药卫生—中药学]