人工虫草与冬虫夏草HPLC特征图谱研究  

Comparative Research on HPLC Characteristic Fingerprint of Cultured Cordyceps Militarise and Cordyceps Sinensis

作　　者：张薇薇 龚韬 韩东河 

机构地区：[1]北京市卫生局临床药学研究所,北京100035

出　　处：《中国中医药信息杂志》2015年第8期92-96,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市中医药科技发展基金项目(JJ2009-14)

摘　　要：目的 以人工北虫草、发酵虫草菌粉和冬虫夏草为研究对象,建立高效液相色谱（HPLC）特征图谱.方法 采用超声法提取虫草中的水溶性成分,HPLC 建立虫草的特征图谱.采用Agilent ZORBAX SB-Aq 色谱柱（250 mm×4.6 mm,5 μm）,流动相为乙腈（A）-0.04 mol/L 磷酸二氢钾溶液（B）,梯度洗脱（0～16 min,0%A;16～38 min,0%→10%A;38～58 min,10%→15%A;58～65 min,15%→0%A）,流速1.0 mL/min,柱温25 ℃,检测波长260 nm,进样体积20 μL.运用国家药典委员会《中药色谱指纹图谱相似度评价系统（2004 A）》,建立人工虫草与天然虫草HPLC 特征图谱,同时指认了7 个核苷类成分,并进行比较研究.结果 本方法精密度、重复性、稳定性良好.以腺苷为参照峰,对11 批冬虫夏草、10 批人工北虫草和12 批发酵虫草菌粉进行指纹图谱分析结果显示,其指纹图谱相似度分别为0.889～0.982、0.781～0.980、0.502～0.970.结论 天然虫草的指纹图谱相似度较高,人工北虫草和发酵虫草菌粉指纹图谱的相似度较低.本方法可作为评价人工虫草和天然虫草质量的参考依据.Objective To establish fingerprints of cultured Cordyceps Militaris, CordycepsSinensis and Fermental Cordyceps. Methods Water soluble components in Cordyceps Sinensiswere extracted by ultrasonic method. The characteristic fingerprints were established by HPLC,with Agilent ZORBAX SB-Aq （4.6 mm × 250 mm, 5 μm） as column and acetonitrile （A）-0.04 mol/LKH2PO4 （B） as mobile phase;gradient elution （0-16 min, 0%A;16-38 min, 0→10%A;38-58 min,10%→15%A;58-65 min, 15→0%A）;the flow rate was 1.0 mL/min;column temperature was25 ℃;the detection wavelength was 260 nm;the injection volume was 20 μL. The HPLCcharacteristic fingerprint of mycelium was developed, and the components of adenosine, uridine,guanosine, inosine, uracil, adenine and cordycepin were identified and compared. Results Thismethod is with high degree of precision, good repeatability and stability. With adenosine asreference peak, similarity of 11 batches of Cordyceps Sinensis collected from different habitats, 10batches of cultured Cordyceps Militarise and 12 batches of Fermental Cordyceps were 0.889-0.982, 0.781-0.980 and 0.502-0.970, respectively. Conclusion There were good similarities betweenthe standard fingerprint and each fingerprint of the eleven samples of Cordyceps Sinensis. Therewere low similarities between the standard fingerprint and each fingerprint of the ten samples ofcultured Cordyceps Militaris and the twelve samples of Fermental Cordyceps. This method can bereference base for the evaluation of quality of cultured Cordyceps Militaris and CordycepsSinensis.

关 键 词：人工北虫草 发酵虫草菌粉 冬虫夏草 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]