调心安神针法对室性心律失常模型兔心肌组织β_2肾上腺素能受体的影响  

Effects of Tiaoxin Anshen acupunture on β_2-adrenergic receptors in rabits with ventricular arrhythmia

作　　者：王锐[1] 郭丽[1] 李永春[2] 崔华峰[1] 韩晶[1] 王长春[1] 李承家[1] 安娜[2] 谢森[2] 

机构地区：[1]山东中医药大学附属医院,山东济南250011 [2]山东中医药大学,山东济南250011

出　　处：《中医临床研究》2015年第16期29-30,共2页Clinical Journal Of Chinese Medicine

基　　金：国家中医药管理局针灸重点学科(山东中医药大学附属医院)重点项目

摘　　要：目的:研究调心安神针法对于室性心律失常模型兔心肌组织β2肾上腺素能受体(ADRB2)蛋白表达的影响。方法:采用兔耳缘静脉注射氯化钡的方法制作室性心律失常模型。将40只新西兰兔随机分为正常组、模型组、针刺组、药物组,针刺组施以调心安神针刺法(取穴:灵台、神道、双侧内关、百会),药物组给予心律平灌胃。实验完毕后取兔左心室,免疫印迹试验技术检测ADRB2蛋白表达水平。结果:模型组的ADRB2蛋白表达较对照组有增加的趋势,药物组及针刺组均明显低于对照组及模型组,药物组的ADRB2蛋白表达低于针刺组。结论:调节心肌组织β2肾上腺素能受体表达可能是该针法治疗心律失常的靶点之一。Objective: To observe effects of the Tiaoxin Anshen acupuncture on the expression ofβ2-adrenergic receptors (ARDB2) protein in cardiac muscle of rabbits with ventricular arrhythmia. Methods: 40 rabits were randomly divided into four groups: the control group, the model group, the acupuncture group and the propafenone group. The acupuncture group were treated by the Tiaoxin Anshen acupuncture (points selected: Lingtai, Shendao, Shuangce Neiguan and Baihui). And the propafenone group were treated with the propafenone by intragastric. Left ventricle of all the rabbits were taken out to test the expression ofβ2-adrenergic receptors (ARDB2) protein by western blot. Results: Expression of ADRB2 proterin was increased in rabbits of the model group than the normal group, decreased in rabbits of the propafenone group and the acupuncture group than the control group and the model group. Expression of ADRB2 protein in the propafenone group was lower than the acupuncture group. Conclusion: Maybe regulating the expression of ARDB2 protein was one of the therapeutic target spots of the Tiaoxin Anshen acupuncture on ventricular arrhythmia.

关 键 词：调心安神针法 室性心律失常 Β2肾上腺素能受体 免疫印迹试验 

分 类 号：R245.3[医药卫生—针灸推拿学]