C57BL/6小鼠动脉粥样硬化中CD4^+CD25^+Treg和TGF-β的表达及其意义  

Expression and significance of CD4^+CD25^+Treg cells and TGF- β in C57BL /6 mice model of atherosclerosis

作　　者：陈越峰[1] 陶琳琳[1] 王庆航[1] 朱洁[1] 周碧蓉[1] 

机构地区：[1]安徽医科大学第一附属医院心血管内科,合肥230022

出　　处：《安徽医科大学学报》2015年第7期888-891,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽医科大学第一附属医院博士启动基金;安徽省高校省级自然科学研究重点项目(编号:KJ2011A158)

摘　　要：目的通过建立C57BL/6小鼠动脉粥样硬化模型探讨CD4+CD25+调节性T细胞(Treg)在动脉粥样硬化发病及治疗过程中的作用。方法 24只雄性C57BL/6小鼠随机分为3组:正常对照组、动脉粥样硬化组、阿托伐他汀组。正常对照组给予基础饲料,其余组给予高脂饲料。高脂饲养4周后,阿托伐他汀组继续高脂饲养并加用阿托伐他汀干预,3组共观察16周。苏木精染色观察血管病变情况,流式细胞仪检测外周血CD4+CD25+Treg的表达情况,ELISA法检测外周血TGF-β浓度。结果 1动脉粥样硬化组的动脉血管的内膜显著增生,CD4+CD25+Treg/CD4+T细胞比例显著低于正常对照组,差异有统计学意义(P<0.01)。阿托伐他汀组的动脉血管较动脉粥样硬化组病变明显减轻。阿托伐他汀组的CD4+CD25+Treg/CD4+T细胞比例较动脉粥样硬化组有显著提升(P<0.01),与正常对照组差异无统计学意义。2动脉粥样硬化组TGF-β浓度较正常对照组明显降低(P<0.01)。阿托伐他汀组的TGF-β的浓度显著高于动脉粥样硬化组(P<0.01);较正常对照组略低,差异无统计学意义。结论 CD4+CD25+Treg及其分泌的TGF-β具有抑制动脉粥样硬化发展的作用,有望成为治疗动脉粥样硬化的新靶点。Objective To investigate effects and mechanism on CD4^＋CD25^＋Treg in C57 BL/6 mice model of ath-erosclerosis. Methods Twenty-four male C57BL/6 mice were assigned to three groups：control group, atherosclero-sis group and atorvastatin group. Control group was given normal diet, and the rest groups were given high fat diet. After 4 weeks of high fat diet,atorvastatin group was continued to keep high fat diet and given drugs. The mice were observed for 16 weeks. Vasculopathy was analyzed by hematoxylin-eosin staining. Expression of CD4^＋CD25^＋Treg in peripheral blood was analyzed by flow cytometry. Concentration of TGF-β was analyzed by ELISA. Results ①Hyperplasia of intima was observed in AS group, and the percentage of CD4^＋CD25^＋Treg/CD4^＋ T cell was signifi-cantly lower than that of the control group（P〈0. 01）. Atatorvastatin could significantly reduce the intimail thick-ness. The percentage of CD4^＋CD25^＋Treg/CD4^＋ T cell was significantly higher than that of AS group（P〈0. 01）. Atorvastatin group and control group had no significant difference.②As group′s concentration of TGF-βwas signif-icantly lower than the control group（P〈0. 01）. Atorvastatin group′s concentration of TGF-βwas significantly high-er than that of AS group（P〈0. 01）. Atorvastatin group and control group had no significant difference. Conclu-sion Our date provides evidence to show CD4^＋CD25^＋Treg and autocrine secretion of TGF-β can inhibit the de-velopment of atherosclerosis,and they will be a new therapeutic target of atherosclerosis.

关 键 词：调节性T细胞 动脉粥样硬化 流式细胞仪 阿托伐他汀 

分 类 号：R541.4[医药卫生—心血管疾病]