C57BL/6小鼠放射性心脏损伤动物模型的建立及血清标志物的探讨  

Establishment of irradiation induced heart injury in C57 mice model and identification of serum marker

作　　者：符天晓[1] 周菊英[1] 焦旸[2] 

机构地区：[1]苏州大学附属第一医院肿瘤放疗科,苏州215006 [2]苏州大学放射医学与公共卫生学院,苏州215006

出　　处：《安徽医科大学学报》2015年第7期922-925,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家青年自然基金项目(编号:81302382)

摘　　要：目的探讨放射性心脏损伤动物模型建立方法和相关血清标志物。方法雌性C57BL/6小鼠28只,随机分为3组:对照组(4只),18 Gy放疗组(12只),25 Gy放疗组(12只)。每周测小鼠体重,分别于照射后第0、8、16周,取心肌组织行HE染色,观察心脏病理组织改变并进行评分;采集下腔静脉血检测心肌肌钙蛋白I(c Tn I)。结果与对照组比较,放疗组小鼠的体重开始呈下降趋势后缓慢上升。放疗组小鼠的心肌组织存在明显的组织病理学改变,早期以急性炎症为主,后期以进行性纤维化为特征;随着受照剂量的增加,炎性反应和纤维化程度加重,病变时间提前。c Tn I随时间变化呈升高趋势,和剂量大小、病理学表现均呈相关性。结论成功建立放射性心脏损伤动物模型。c Tn I可作为评价心脏损伤动物模型的无创性指标。Objective To establish and investigate C57 BL/6 mice model of radiation induced heart injury and ser-um marker. Methods Twenty eight female C57BL/6 mice were randomly divided into 3 groups：normal group （n=4 ） , 18 Gy radiation therapy group （ n=12 ） , 25 Gy radiation therapy group （ n=12 ） . The mice were weighed every week. Respectively, 0, 8 and 16 weeks after irradiation, the pathological changes of myocardial tissue were observed by hemetoxylin-eosin（ HE） staining and made pathological score. Inferior vena cava blood was collected to take cardiac troponin I （ cTnI） test. Results Compared with the normal group,the weight of radiation group went down first then went up slowly. The myocardial tissue from mice had obvious histopathological changes. Acute in-flammation was the main change in the early days. At the late stage, progressive fibrosis was the main characteris-tic. With the increase of the dose, the inflammatory reaction and fibrosis degree had aggravated, pathological chan-ges had occurred earlier. cTnI showed a trend of higher performance over time, and had a correlation with patholo-gy. Conclusion We successfully established radiation induced myocaridal injury model. As a noninvasive serum marker, cTnI can be used as the evaluation standard of radiation induced myocardial injury animal model.

关 键 词：放射性心脏损伤 动物模型 评价方法 肌钙蛋白 

分 类 号：Q95-3[生物学—动物学] R818.74[医药卫生—放射医学]