rhEPO对新型BPD模型肺组织BCL-2和Caspase-9表达的影响及意义  

The effect and significance of rh EPO to the expression of BCL- 2 and Caspase-9 in the new type BPD model's lung tissue

作　　者：岳嗣凤 张华[1] 

机构地区：[1]桂林医学院附属医院新生儿科,桂林541001

出　　处：《安徽医科大学学报》2015年第7期933-936,共4页Acta Universitatis Medicinalis Anhui

基　　金：广西医疗卫生重点科研课题(编号:重2011011)

摘　　要：目的探讨磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/AKT)通路在重组人促红细胞生成素(rh EPO)保护新型支气管肺发育不良(BPD)中的作用。方法 40只定期受孕的SD大鼠,于孕第15天孕囊内注射脂多糖(LPS)或磷酸盐缓冲液(PBS),新生大鼠分为4组:对照组、高氧组、rh EPO组、rh EPO+LY294002组。采用Western blot法和RT-PCR法检测肺组织中BCL-2和Caspase-9蛋白及mRNA表达。结果高氧组、rh EPO+LY294002组新生大鼠肺组织BCL-2蛋白及mRNA表达减少,Caspase-9蛋白及mRNA表达明显增加;rh EPO组BCL-2蛋白及mRNA表达增加,Caspase-9蛋白及mRNA表达减少;于高氧暴露第1、7、14天各组间的差异均有统计学意义(P<0.05)。结论宫内炎性暴露联合生后高氧可导致肺细胞凋亡增加,rh EPO可能通过减少肺细胞凋亡,对新型BPD起一定的保护作用,其机制可能与PI3K/AKT信号转导通路有关。Objective To explore the role of phosphatidylinositol 3-kinase/protein kinase B（PI3K/AKT） pathway in recombinant human erythropoietin（ rhEPO） protecting new type BPD. Methods Injected LPS or PBS to 40 spra-gue-dawley （SD） rats, gestational sacs on 15th day of gestation, the newborn rats were divided into four groups：control group, hyperoxia group, rhEPO group, rhEPO＋LY294002 group. To detect the expression of lung tissue BCL-2 and Caspase - 9 protein and mRNA by Western blot and RT-PCR. Results In the hyperoxia group and rhEPO＋LY294002 group, BCL-2 expression was decreased,while Caspase-9 expression was increased apparently;in the rhEPO group, BCL-2 expression was increased, while Caspase-9 expression was decreased. The differences between each group were statistically significant on 1st,7th and 14th day of hyperoxia exposure （P〈0. 05）. Con-clusion Intrauterine inflammatory exposure combined with hyperoxia after birth can cause lung cell apoptosis in-creases. While rhEPO has certain control effects on BPD, possibly by reducing the pulmonary apoptosis. The mechanism may be associated with PI3K/AKT signaling pathway.

关 键 词：支气管肺发育不良 重组人促红细胞生成素 细胞凋亡 磷脂酰肌醇3-激酶/蛋白激酶B 

分 类 号：R332[医药卫生—人体生理学]