TLR4 mAb对人正常肝内胆管上皮细胞LPS-TLR4-NF-κB通路的影响  

Effect of TLR4 mAb on the normal intrahepatic of bile duct epithelial cells of human LPS-TLR4-NF-κB pathway

作　　者：刘辉[1] 余宏铸[1] 韩珑[1] 王正林[1] 

机构地区：[1]安徽医科大学第一附属医院肝胆外科,合肥230022

出　　处：《安徽医科大学学报》2015年第7期946-949,共4页Acta Universitatis Medicinalis Anhui

基　　金：2013年安徽省年度重点科研计划项目(编号:1301043034)

摘　　要：目的对脂多糖(LPS)及Toll样受体4单克隆抗体(TLR4 mA b)作用于体外培养的人肝内胆管细胞(HiB EC)后其TLR4 mRNA和核转录因子-κB(NF-κB)mRNA的表达情况进行了研究。方法体外培养HiB EC,采用RT-PCR法检测经LPS和不同浓度的TLR4 mA b作用后,HiB EC细胞株中TLR4 mRNA和NF-κB mRNA的表达情况。结果 LPS可以上调HiB EC中TLR4 mRNA和NF-κB mRNA的表达(P<0.05);而LPS作用于TLR4 mA b处理后的HiB EC中其TLR4 mRNA和NF-κB mRNA的表达下调;高质量浓度TLR4mA b使NF-κB mRNA的表达下调更明显(P<0.05)。结论LPS能上调HiB EC中TLR4 mRNA和NF-κB mRNA的表达;而TLR4 mA b则能拮抗LPS对HiB EC的刺激作用,下调TLR4 mRNA和NF-κB mRNA的表达。Objective To observe the expression of Toll-like receptor 4 （TLR4）, nuclear factor-κB （NF-κB） in-duced by lipopolysaccharide （ LPS） and Toll-like receptor 4 monoclonal antibodies （ TLR4 mAb） in normal human intrahepatic biliary epithelial cells （ HiBEC） in vitro. Methods We cultured HiBEC in vitro. The cells were stim-ulated by LPS and different concentrations of TLR4 mAb. The levels of mRNA expression of TLR4 and NF-κB were detected by reverse transcription PCR （ RT-PCR） . Results LPS increased the mRNA expression of TLR4 and NF-κB （ P〈0. 05 ） . TLR4 mAb reduced the mRNA expression of TLR4 and NF-κB （ P〈0. 05 ） after LPS-stimulated HiBEC. High concentrations of TLR4 mAb reduced the mRNA expression of NF-κB （P〈0. 05） after LPS-stimula-ted HiBEC in vitro. Conclusion The mRNA expression of TLR4 and NF-κB is increased afer LPS-stimulated Hi-BEC. TLR4 mAb antagonize the role of LPS on TLR4 and NF-κB,and reduce the mRNA expression of TLR4 and NF-κB.

关 键 词：脂多糖 Toll样受体4单克隆抗体 TOLL样受体4 核转录因子-ΚB 

分 类 号：R349.54[医药卫生—基础医学]