桂林市经性传播的HIV-1的流行特征和亚型分析  

Epidemiology and gene analysis of HIV-1 infections in Guilin who infected by sexually transmission

作　　者：孔衍琳 张帅[1] 王雪雯[1] 李赫伟[1] 胡婷婷[1] 蒋骞[1] 蒋就喜[1] 潘定权[2] 张振开 王海泉 李林[4] 孙常荣[4] 李敬云[4] 梁浩[5] 梁冰玉[5] 张奇[6] 

机构地区：[1]桂林医学院附属医院感染性疾病科,桂林541001 [2]桂林市疾病预防控制中心,桂林541001 [3]桂林市传染病医院,桂林541002 [4]军事医学科学院微生物流行病研究所,北京100071 [5]广西医科大学公共卫生学院,南宁530021 [6]临沂市沂水中心医院,临沂276000

出　　处：《安徽医科大学学报》2015年第7期966-969,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然基金(编号:81273137);国家科技重大专项课题(编号:2012ZX10001-002);广西本级技术研究与开发项目(桂财教[2012]98号);桂林市科学研究与技术开发项目(编号:20130120-18);广西壮族自治区研究生教育创新计划资助项目(编号:YCSZ2013101)

摘　　要：目的 了解桂林市2008年及以后确诊的经性传播的人类免疫缺陷病毒1型（HIV-1）感染者的流行病学及亚型的分布情况。方法 选取样本100例,收集流行病学资料,采集抗凝全血,提取病毒RNA,RT-PCR法行gag基因扩增,对产物进行测序和分析。结果 100例样本中男女比例为2.125∶1;青壮年年龄组（20~49岁）患者为54%,〉50岁年龄组患者为45%;职业为农民的患者为40%,学生为6%;初中及以下文化水平患者占69%;经异性和同性性接触途径传播的分别为93%、7%。100例样本最终成功扩增得到61例样本的gag基因序列,CRF01-AE重组亚型48例,CRF07-BC重组亚型8例,B亚型5例。结论 应更加重视婚检、孕检的艾滋病抗体筛查;关注老年人群经性接触途径感染艾滋病的现象;采取针对低素质人群的有效防治措施;随着主要传播途径和主要亚型的变化,迫切需要对高危人群进行宣传教育和行为干预以阻断HIV/AIDS的流行和传播。Objective To determine the epidemiological situation of confirmed HIV-1 infections in Guilin after 2008 who were infected by sexually transmission, and to understand the distribution of HIV-1 subtypes. Methods 〈br〉 To make epidemiological survey on the 100 confirmed HIV-1 infections, extract HIV-1 RNA from their whole blood and amplified by RT-PCR. HIV-1 gag gene was sequenced directly from the PCR product. Results In the chosen patients, male to female ratio was 2. 125 ∶ 1. Patients aged ≥20 and ≤49 years old accounted for 54% in total, and patients aged 50 years old or older accounted for 45%. Farmers accounted for 40% and students accounted for 6%. Patients with education level of junior middle school or below accounted for 69%. The proportions which were transmitted through heterosexual and homosexual contact were 93% and 7% respectively. 61 specimens were gained in 100 specimens, then sequence analysis showed that there were 3 HIV-1 subtypes, CRF01-AE（48 samples）,B （5 samples）,CRF-07BC（8 samples）. Conclusion The more attentions should be paid to the HIV/AIDS antibody screening in marriage and pregnancy inspection, focus on HIV/AIDS infection though sexual transmission in elderly people, and more effective ways to prevent HIV/AIDS infection for the population with poor education level should be explored. With the change of the main transmission and subtype, the more education and behavioral interven-tions should be done to prevent the spread of HIV/ AIDS.

关 键 词：HIV-1 流行特征 亚型 GAG基因 

分 类 号：R373.51[医药卫生—病原生物学]