生长激素受体在宫腔粘连患者子宫内膜组织中的表达及意义  

Expression and significance of growth hormone receptor in the endometrial tissue of intrauterine adhesion patients

作　　者：任娟[1] 卫兵[1] 宋恩学[1] 王文艳[1] 张丽丽[1] 

机构地区：[1]安徽医科大学第二附属医院妇产科,合肥230601

出　　处：《安徽医科大学学报》2015年第7期1004-1007,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年科学基金项目(编号:81100412)

摘　　要：目的探讨生长激素受体(GHR)在宫腔粘连(IUA)患者子宫内膜组织中的表达及意义。方法通过免疫组化Max Vision两步法和实时荧光定量PCR(qRT-PCR)法分别检测IUA患者(研究组)和非IUA患者(对照组)子宫内膜组织中GHR蛋白和GHR mRNA的表达情况。结果研究组GHR蛋白表达、GHR mRNA和对照组比较差异有统计学意义(P<0.05)。子宫内膜腺体与子宫内膜间质中GHR蛋白表达比较差异有统计学意义(P<0.05)。结论 IUA患者子宫内膜组织中GHR的表达低于非IUA患者,而且这种表达在子宫内膜腺体中较多,在子宫内膜间质中较少,为IUA患者在宫腔粘连分离术术后使用生长激素提供一定的理论参考。Objective To investigate the growth hormone receptor （ GHR） expression and significance of endome-trial tissue in patients with intrauterine adhesions. Methods Immunohistochemistry MaxVision twostep and quanti-tative real-time PCR （ qRT-PCR） were used to detect the expression of GHR protein and GHR mRNA IUA in pa-tients endometrial tissue of IUA group （ study group） and non IUA group （ control group） . Results Using immu-nohistochemistry assay to detect GHR protein expression and qRT-PCR to detect the GHR mRNA expression, and the differences between the study group and the control group were statistically significant（P〈0. 05）. Immunohis-tochemistry was used to detect the expression of GHR protein in endometrial glands and endometrial stromal, the difference between the two parts were statistically significant （ P〈0. 05 ） . Conclusion The expression of GHR in endometrial tissue in patients with IUA is lower than those of non-IUA, and this expression is more in endometrial glands, less in endometrial stroma,which provide a theoretical reference for the use of hormone in IUA patients after transcervical resection of adhesions（ TCRA） .

关 键 词：宫腔粘连 生长激素 生长激素受体 

分 类 号：R711.74[医药卫生—妇产科学]