碳纤维-聚乳酸-聚乙二醇复合支架的制备及性能检测  

Preparation and performance detection of carbon fiber-polylactic acid-polyethylene glycol composite scaffold

作　　者：周常艳[1] 周庆焕[2] 边竟[1] 陈克[1] 陈文[1] 

机构地区：[1]十堰市太和医院(湖北医药学院附属医院)骨科一病区,湖北省十堰市442000 [2]湖北医药学院护理学院,湖北省十堰市442000

出　　处：《中国组织工程研究》2015年第21期3371-3376,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:长期实验发现聚乳酸-聚乙二醇支架的力学性能及细胞相容性能较差,因此多数研究向支架中加入其他材料,以提高其生物活性及力学性能。目的:制备改性碳纤维-聚乳酸-聚乙二醇支架,并检测其性能。方法:采用溶液潘注/粒子沥滤法制备改性碳纤维-聚乳酸-聚乙二醇复合支架。对比改性碳纤维-聚乳酸-聚乙二醇复合支架与聚乳酸-聚乙二醇支架的超微结构、孔隙率、吸水性、降解率及力学性能。将改性碳纤维-聚乳酸-聚乙二醇复合支架与聚乳酸-聚乙二醇支架分别与SD大鼠成骨细胞共培养,12 h后采用沉淀法检测细胞黏附率;培养1,3,5,7,9 d后,采用MTT法检测细胞增殖。结果与结论:聚乳酸-聚乙二醇支架材料表面孔结构分布均匀,孔径为(404.0±10.5)μm;改性碳纤维-聚乳酸-聚乙二醇支架碳纤维表面见大量纵向沟槽,表面孔结构分布均匀,孔径为(433.0±3.0)μm,两组支架孔径比较差异有显著性意义(P<0.05)。改性碳纤维-聚乳酸-聚乙二醇支架的孔隙率、吸水性、弹性模量和抗压强度、降解率、细胞黏附率与增殖率均高于聚乳酸-聚乙二醇支架(P<0.05)。表明改性碳纤维的加入改善了聚乳酸-聚乙二醇复合支架的力学性能及细胞相容性。BACKGROUND：The mechanical performance and cytocompatibility of polylactic acid-polyethylene glycol （PLA-PEG） scaffold is poor via long-term experiments; therefore, other materials are added to the scaffold in order to improve its biological activity and mechanical properties. OBJECTIVE：To investigate the preparation process of carbon fiber （CF）-PLA-PEG scaffold, and to test its performance. METHODS：Modified CF-PLA-PEG composite scaffolds were prepared using solution injection-particle leaching method. Ultra-structure, porosity, water-absorbing quality, degradation rate and mechanical properties of CF-PLA-PEG composite scaffolds and PLA-PEG scaffolds were compared. Rat osteoblasts were co-cultured with these two kinds of scaffoldsin vitro, and the cel adhesion rate was detected by precipitation method after 12 hours. Cel counting was detected using MTT method to determine the cel proliferation at culture days 1, 3, 5, 7, 9. RESULTS AND CONCLUSION：The surface structure of PLA-PEG distributed uniformly, and the aperture was （404.0±10.5） μm. The carbon fiber surface of modified CF-PLA-PEG composite scaffolds had a lot of longitudinal grooves, the surface structure also distributed uniformly, and the aperture was （433.0±3.0） μm. Difference in scaffold aperture was of significance between the two groups （P 〈 0.05）. The porosity, water-absorbing quality, elasticity modulus, compressive strength, degradation rate, cel adhesion rate and cel growth rate of CF-PLA-PEG were better than those of PLA-PEG （P 〈 0.05）. These results show that the CF-PLA-PEG scaffolds have good mechanical property and cel compatibility.

关 键 词：生物材料 骨生物材料 骨组织工程 聚乳酸-聚乙二醇支架 改性碳纤维-聚乳酸-聚乙二醇支架 

分 类 号：R318[医药卫生—生物医学工程]