刺五加皂苷对急性脊髓损伤后脊髓内BDNF和NGF表达的影响  

Effect of acanthopanax senticosus saponins on expression of brain-derived neurotrophic factor and nerve growth factor in rat spinal cord after acute spinal cord injury

作　　者：乔苏迟 陈晓[1] 李诚[1] 刘畅[1] 朱德刚[1] 吾不力.阿扎提 苏佳灿[1] 王志伟[1] 

机构地区：[1]第二军医大学长海医院骨科,上海200433

出　　处：《第二军医大学学报》2015年第6期666-669,共4页Academic Journal of Second Military Medical University

摘　　要：目的研究刺五加皂苷对脊髓损伤后组织内脑源性神经营养因子(BDNF)和脊髓内神经生长因子(NGF)两种蛋白表达的影响,探讨刺五加皂苷对损伤脊髓的保护作用。方法将45只雄性大鼠随机分为3组:假手术组、模型组和刺五加皂苷干预组,每组15只。将实验动物用10%水合氯醛麻醉后,用改良Allen’s法进行急性脊髓损伤模型造模。造模后,假手术组予正常饲养,模型组予连续7d生理盐水腹腔注射,刺五加皂苷干预组进行连续7d100mg/kg刺五加皂苷持续腹腔注射。ELISA法检测血清中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,通过病理切片分析大鼠脊髓组织病理变化,蛋白质印迹法检测大鼠脊髓内BDNF和NGF表达。结果与假手术组比较,模型组和刺五加皂苷干预组脊髓有明显损伤,且大鼠血清中MDA含量增加(P<0.05),SOD活性降低(P<0.05),大鼠脊髓组织中的BDNF和NGF蛋白表达量上升(P<0.05)。与模型组相比,刺五加皂苷组大鼠脊髓损伤明显改善,血清中MDA含量降低(P<0.05),SOD活性升高(P<0.05),大鼠脊髓组织中的BDNF和NGF蛋白表达量上升(P<0.05)。结论刺五加皂苷促进脊髓组织中神经元细胞内BDNF和NGF表达,利于损伤神经元的修复,对大鼠急性损伤的脊髓具有保护作用。Objective To study the effect of acanthopanax senticosus saponins（ASS）on expression of brain-derived neurotrophic factor（BDNF）and nerve growth factor（NGF）in rat spinal cord after spinal cord injury,so as to explore the protective effect of ASS against spinal cord injury.Methods A total of 45 male rats were evenly randomized into three groups：sham operation group,model group and ASS intervention group.The experimental animals were anesthetized with 10%chloral hydrate and then acute spinal cord injury model was made using modified Allen’s method.After modeling the sham group received normal feeding,the model group was intraperitoneally given normal saline for 7consecutive days,and the ASS intervention group was given appropriate dose of ASS for 7consecutive days.Then ELISA was used to detect the serum malondialdehyde（MDA）and superoxide dismutase（SOD）;the pathological changes of spinal cord tissue were observed,and the expression of BDNF and NGF was examined by Western blotting analysis.Results Compared with the sham group,the other two groups had significantly increased serum MDA（P〈0.05）and significantly decreased SOD level（P〈0.05）,with notable spinal cord injury;besides,the two groups also had significantly increased expression of BDNF and NGF protein in the spinal cord tissue（P〈0.05）.Compared with the model group,ASS group had significantly improved spinal cord injury,with significantly decreased serum MDA content（P〈0.05）and increased SOD content and BDNF,NGF expression in the spinal cord tissue（P〈0.05）.Conclusion ASS can promote BDNF and NGF protein expression in neurons of spinal cord,improving repair of damaged neurons and protecting spinal cord against acute injury in rats.

关 键 词：刺五加皂苷 脊髓损伤 氧化性应激 脑源性神经营养因子 神经生长因子 

分 类 号：R651.21[医药卫生—外科学]