尿中亚硫基二乙酸测定的气相色谱-质谱法  

Determination of thiodiglycolic acid in urine with gas chromatography-mass spectrometer

作　　者：肖庆锋 付志华[2] 孟成名 轩月兰 秦文华 

机构地区：[1]河南省职业病防治研究院,郑州450052 [2]郑州大学化学与分子工程学院

出　　处：《中华劳动卫生职业病杂志》2015年第6期474-476,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家重点临床专科建设科研项目（财社[2011]170）

摘　　要：目的 建立氯乙烯（VCM）生物标志物亚硫基二乙酸（TDGA）的气质联用仪（GC-MS）快速检测方法.方法 以温和新型甲酯化反应替代剧毒重氮甲酯化反应,并建立高灵敏度检测方法.结果 该方法标准曲线回归线性方程：y=8 460.5x-4758.2,线性系数为0.999 7,最低定量浓度2.0 μg/L,精密度为0.81％～2.38％,平均加标回收率为99.0％～102.9％.结论 该方法降低了传统甲酯化反应的风险,较气相色谱-火焰光度检测器（GC-FPD）法提高了检测灵敏度,满足亚硫基二乙酸的检测要求.Objective To establish a rapid determination method with gas chromatography-mass spectrometer （GC-MS） for thiodiglycolic acid （TDGA）,a vinyl chloride （VCM） biomarker.Methods A high-sensitivity determination method was established using a moderate methyl esterification instead of methyl esterification of highly toxic diazo reaction.Results The standard curve regression linear equation of the method was：y=8460.5x-4758.2,the linear coefficient was 0.999 7,the minimum quantity concentration was 2.0 μg/L,the range of precision value was 0.81%～2.38%,and the average recovery of standard addition was 99.0%～ 102.9%.Conclusion This method reduces the risk of traditional methyl esterification,improves the determination sensitivity compared with the GC-FPD method,and meets the determination requirement of TDGA.

关 键 词：氯乙烯 生物标志物 亚硫基二乙酸 色谱法 气相-质谱 

分 类 号：R446.12[医药卫生—诊断学] O657.63[医药卫生—临床医学]