The functional analysis of OsTDF1 reveals a conserved genetic pathway for tapetal development between rice and Arabidopsis  

作　　者：Ci-Feng Cai Jun Zhu Yue Lou Zong-Li Guo Shuang-Xi Xiong Ke Wang Zhong-Nan Yang 

机构地区：[1]Collegeof Life and Environment Sciences, Development Centerof Plant Germplasm Resources, Shanghai Normal University,Shanghai 200234, China

出　　处：《Science Bulletin》2015年第12期1073-1082,I0002,共11页科学通报（英文版）

基　　金：This work was supported by the grants from the Ministry of Science and Technology of China （2013CB945100）, the National Natural Science Foundation of China （31100227）.

摘　　要：During anther development, the tapetum pro- vides materials and nutrients for pollen development. In Arabidopsis, several transcription factors have been iden- tified to form a genetic pathway （DYT1-TDF1-AMS- MS188-MS1） for tapetal development and function. DEFECTIVE in TAPETAL DEVELOPMENT and FUNCTION1 （TDF1） is a member of the R2R3 MYB family and is essential for early tapetum development in Arabidopsis. Here, we characterized an ortholog of Ara- bidopsis TDF1 in rice, OsTDF1 （LOC_Os03g18480）. OsTDF1 shares 69 % amino acid sequence identity with AtTDF1 in the putative MYB domain near the N-terminal region. RT-PCR and in situ hybridization show that OsTDF1 is specifically expressed in tapetal cells of rice anthers. The expression of OsTDF1 in Arabidopsis tdfl mutant restores its fertility, suggesting that this homolog can fulfill the normal function of TDF1 in Arabidopsis. The ostdfl knockout mutant exhibits a male-sterile phenotype. Its tapetal cells exhibit a vacuolated and hypertrophic phenotype similar to that of Arabidopsis tdfl mutants. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assays revealed OsTDF1 acts as an essential regulator for tapetum programmed cell death. The qRT-PCR analysis revealed that OsTDF1 acts downstream of UDT1 and upstream of TDR, EAT1, OsMYBI03 and PTCI in rice, suggesting that the genetic pathway for tapetum development is generally conserved between rice and Arabidopsis,在花药开发期间， tapetum 为花粉开发提供材料和营养素。在 Arabidopsis，几个抄写因素被识别了为 tapetal 开发和功能形成一条基因小径(DYT1TDF1AMSMS188MS1 ) 。在 TAPETAL 开发和 FUNCTION1 (TDF1 ) 的有缺陷者是 R2R3 MYB 家庭的一个成员并且为在 Arabidopsis 的早 tapetum 开发是必要的。这里，我们在米饭描绘了 Arabidopsis TDF1 的 ortholog， OsTDF1 (LOC_Os03g18480 ) 。OsTDF1 分享 69 ? 有在 N 终端区域附近的通常认为的 MYB 领域的 AtTDF1 的 % 氨基酸顺序身份。RT-PCR 并且在 situ 杂交表演，那 OsTDF1 明确地在米饭花药的 tapetal 房间被表示。在 Arabidopsis tdf1 异种的 OsTDF1 的表达式恢复它的富饶，建议这个相当或相同的事物能完成在 Arabidopsis 的 TDF1 的正常函数。ostdf1 大美人异种展出男性无菌的显型。它的 tapetal 房间展出类似于 Arabidopsis tdf1 异种的 vacuolated 和 hypertrophic 显型。终端 deoxynucleotidyl 当为 tapetum 的一个必要管理者规划了房间死亡，把试金标记的调停 transferase 的 dUTP 刻痕结束揭示了 OsTDF1 行为。qRT-PCR 分析表明 OsTDF1 UDT1 下游地行动，建议为 tapetum 开发的基因小径通常在米饭和 Arabidopsis 之间被保存并且在米饭的 TDR， EAT1， OsMYB103 和 PTC1 在上游。

关 键 词：Oryza sativa Transcription factor TAPETUM OsTDF1 

分 类 号：Q943[生物学—植物学] S572.1[农业科学—烟草工业]