高温胁迫对甜椒幼苗生长和生理生化指标的影响  

Effects of high temperature stress on the growth and physiological and biochemical indexes of sweet pepper seedlings

作　　者：刘凯歌[1,2] 宋云鹏[1] 龚繁荣[1] 朱月林[2] 郝婷[2] 

机构地区：[1]上海市农业科学院园艺研究所上海市设施园艺技术重点实验室,上海201403 [2]南京农业大学园艺学院,南京210095

出　　处：《上海农业学报》2015年第3期63-67,共5页Acta Agriculturae Shanghai

基　　金：上海市科学技术委员会科研计划项目(13391901100)

摘　　要：以2种耐热性不同的甜椒品种‘越夏2198'和‘:P13201'为材料,研究了高温条件下(昼40℃夜30℃)丙二醛(MDA)含量、相对电导率、脯氨酸(Pro)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性以及甜椒生长指标的变化趋势,为探讨甜椒耐高温胁迫的生理机制以及为甜椒苗期耐热性鉴定提供参考。结果显示:(1)高温胁迫下,甜椒幼苗的生长受到抑制,2个品种的株高、茎粗、地上鲜重、地下鲜重均呈下降趋势,热敏品种的各生长指标下降幅度显著高于耐热品种,恢复效果也较耐热品种差;(2)2个品种的MDA含量和相对电导率在高温处理期间均呈逐渐升高趋势,并且热敏品种始终高于耐热品种;脯氨酸含量在高温胁迫期间的表现趋势和MDA含量类似,但耐热品种显著高于热敏品种,于高温第5天两者差异达到最大;(3)高温胁迫导致两个品种的SOD和POD活性均呈先升后降趋势,但耐热品种始终高于热敏品种,POD活性表现尤为明显。结果表明:高温条件下,2个甜椒品种的相同指标在相同处理天数下存在差异,并且上升或下降幅度也有所不同。这种差异性为阐明甜椒耐热机理和甜椒耐热遗传育种提供了理论参考。In order to elucidate the physiological and biochemical mechanism of heat-tolerant sweet pepper and provide a reference for heat resistance identification of sweet pepper at seedling stage,the seedling growth of 2 sweet pepper varieties（‘Yuexia 2198’and ‘P13201’）with different heat tolerances was studied in high tem-perature conditions（40 ℃ at day and 30 ℃ at night）in terms of malondialdehyde（MDA）content,relative elec-tric conductivity,proline content,superoxide dismutase（SOD）activity,peroxidase（POD）activity and growth indi-ces.The results showed that（1）the seeding growth was inhibited under high temperature stress,the 2 varieties’ plant height,stem diameter,fresh shoot weight and fresh root weight were decreased,but the heat-sensitive variety had a larger decline in all the growth indexes and a poorer restoration result;（2）The 2 varieties’MDA content and relative electric conductivity increased during high temperature stress,and the heat-sensitive variety’s were higher than the heat-resistant variety’s all the time;the 2 varieties’proline content had a similar result to their MDA content,but the heat-resistant variety’s was significantly higher than the heat-sensitive variety’s,and their difference reached maximum on the 5th day under high temperature stress;（3）The 2 varieties’SOD and POD activities increased at first and then decreased,but the heat-sensitive variety’s were less than the heat-resistant variety’s all the time,especially the POD activity.The above results indicated that under the same heat treatment there were differences in the same indicators between the 2 varieties,and their increments or decrements were al-so different.Those differences provide a theoretical basis for elucidation of heat resistance mechanism and genetic breeding of sweet pepper.

关 键 词：甜椒 抗热性 生理指标 高温胁迫 温室栽培 

分 类 号：S512.1[农业科学—作物学]