三种经典钙黏蛋白分子在鸡胚脊髓发育过程中的表达模式  

Expression patterns of three classical cadherins in the spinal cord during chicken embryonic development

作　　者：李晗[1] 杨慈清[1,2] 乔梁[1,2] 张必超[1] 于亚楠[1] 林俊堂[1,2] 

机构地区：[1]新乡医学院生命科学技术学院,新乡453003 [2]新乡医学院河南省医用组织再生重点实验室,新乡453003

出　　处：《解剖学杂志》2015年第3期261-264,F0003,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金(31440049);新乡医学院2013年研究生科研创新支持计划资助项目(YJSCX201312Z);新乡医学院科研培育基金(2013QN125)

摘　　要：目的：揭示神经钙黏蛋白、钙黏蛋白6与钙黏蛋白7在鸡胚脊髓发育过程中的时空表达模式。方法：取鸡胚正常发育过程 E3、E4、E5、E6、E8和 E10各时期的脊髓,原位杂交法检测神经钙黏蛋白、钙黏蛋白6与钙黏蛋白7在鸡胚脊髓发育过程中的时空表达模式。结果：在 E3~E6时期,神经钙黏蛋白主要在神经上皮层、生皮节、脊索、顶板、底板以及背根神经节区域表达,E8~E10时期,其 mRNA 信号主要在灰质区和背根神经节；在 E3~E5时期,钙黏蛋白6主要在生皮节、顶板和底板表达,之后在顶板和底板的表达均减弱,主要在侧运动柱表达；E3~E6时期,钙黏蛋白7主要在生皮节、底板、基板背部、运动柱、背根和腹根强烈表达,E8之后其表达主要集中在灰质区和腹根。结论：神经钙黏蛋白、钙黏蛋白6与钙黏蛋白7不同的表达模式预示着它们在脊髓及其周围组织发育过程中扮演不同角色。Obj ective ：To clarify the regulated spatiotemporal expression patterns of N-cadherin,cadherin-6 and cadherin-7 in the spinal cord during chicken embryonic development.Methods ：Spinal cords at E3,E4,E5 ,E6,E8 and E10 were collected during chicken embryonic development.The expression patterns of N-cadherin,cadherin-6 and cadherin-7 were investigated with in situ hybridization in the spinal cord at different embryonic stages.Results ：From E3 to E6,the signal of N-cadherin was detected in the neuroepithelium,dermatome,notochord,roof plate,floor plate and dorsal root ganglion.It was mainly expressed in the gray matter from E8 to E10.Cadherin-6 was mainly expressed in the dermatome,roof plate and floor plate from E3 to E5 .Then the positive signal was mainly exhibited in the motor column.Cadherin-7 was mainly expressed in the dermatome,floor plate,dorsal domain of the basal plate,motor column,and dorsal and ventral roots at early stages. Cadherin-7 was mainly expressed in the gray matter and ventral root from E8 to E10.Conclusion：Different expression patterns of N-cadherin,cadherin-6 and cadherin-7 mean their diverse roles in the spinal cord and its surrounding tissues during chicken embryonic development.

关 键 词：神经钙黏蛋白 钙黏蛋白6 钙黏蛋白7 原位杂交 表达模式 

分 类 号：R338[医药卫生—人体生理学]