雌激素受体-β基因多态性及山茶籽油与广西壮族绝经后骨质疏松症的关联性  

Correlation of polymorphism of estrogen receptor-β and camellia oil with post-menopausal osteoporosis in Zhuang women of Guangxi

作　　者：黄海玲[1] 谭慧欢[2] 陈秉朴[1] 李海[1] 解继胜[1] 赵琼芝[1] 

机构地区：[1]右江民族医学院 [2]百色市人民医院,百色533000

出　　处：《解剖学杂志》2015年第3期323-325,343,共4页Chinese Journal of Anatomy

基　　金：广西自然科学基金(2010GXNSFA013268、2013GXNSFAA019241);2013年校地校企共建科技创新平台立项建设项目-桂西地区少数民族基因多态性研究平台;广西中医药科技专项课题(CZPT13-47);广西教育厅科研项目(200103YB115);广西教育厅立项课题(201106LX432、2013LX105)

摘　　要：目的：探索雌激素受体-β（ER-β）基因多态性及山茶籽油与广西壮族妇女绝经后骨质疏松症（PMOP）的关联性。方法：590例广西长期食用山茶籽油壮族妇女和600例广西长期非食用山茶籽油壮族妇女,定量超声骨质密度仪检测2组右侧足跟骨测量宽带超声衰减值（BUA）,PCR-RFLP 检测2组 ER-β基因多态性。结果：同一年龄组相比较,食用山茶籽油组绝经妇女骨质疏松症的患病率明显低于非食用山茶籽油组绝经妇女。ER-β基因 RsaⅠ等位基因频率在2组中存在着显著性差异,r 等位基因患 PMOP 的风险是 R 等位基因的1.442倍（OR=1.442,95%CI 为1.215~1.711）。食用山茶籽油组绝经后骨质疏松症组携带 AluⅠ酶切位点的 a 等位基因分布频率明显少于非食用山茶籽油组绝经后骨质疏松症组。结论：提示 ER-βRsaⅠ酶切位点的 r 等位基因可能是 PMOP 的遗传易感基因；食用山茶籽油对 PMOP 有防治作用,AluⅠ酶切位点的 a 等位基因是对山茶籽油起交互作用的敏感基因。Obj ective ：To explore the correlation of polymorphism of estrogen receptor-β（ER-β）and camellia seed oil with post-menopausal osteoporosis （PMOP）in Zhuang women of Guangxi.Methods ：5 9 0 and 6 0 0 Zhuang women,who ingested regularly or nerve intook camellia oil （ECO）in long-term （grouped as ECO and NECO correspondingly）,underwent right calcaneal broad band ultrasound attenuation （BUA） analysis by using quantitative ultrasound bone densitomerter,ER-βpolymorphism detection using PCR-RFLP.Results ：In the same age subgroup,the prevalence of PMOP in ECO group was significantly lower than that in NECO group.The difference in ER-βgene Rsa Ⅰ allele frequencies was found in the above two groups.And,risk for PMOP with allele r was 1 .4 4 2 times than that of R allele （OR= 1 .4 4 2 ,9 5 % CI：1 .2 1 5 1 .7 1 1 ）. The frequency of Alu Ⅱ restriction a allele occurred in ECO women with PMOP was significantly less than that in NECO women with PMOP.Conclusion：The ER-β r allele gene may be a genetic susceptibility gene of PMOP;intake of camellia seed oil has preventive and therapeutic effect on PMOP, and a allele may be a sensitive gene in the interaction with camellia oil.

关 键 词：雌激素受体-Β 多态性 绝经后骨质疏松症 壮族 

分 类 号：R580[医药卫生—内分泌]