金标免疫层析读数仪在棘球蚴病抗体检测中的应用  

The Application of Gold-immunochromatographic Test Strip Reader in Serum Antibody Detection in Echinococcosis

作　　者：谢贵林[1] 殷军霞 段新宇 冯晓辉 王洋[4] 蒋凯[4] 陈新梅[5] 

机构地区：[1]绍兴第二医院肝胆外科,绍兴312000 [2]湖北省天门市卫生与计划生育委员会,天门431700 [3]新疆维吾尔自治区包虫病临床研究所,乌鲁木齐830011 [4]浙江大学生物医学工程与仪器科学学院,杭州310013 [5]山东中医药大学药学院,济南250355

出　　处：《中国寄生虫学与寄生虫病杂志》2015年第3期211-213,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.81372425);浙江省自然科学基金(No.LY13H180003);新疆科技厅合作项目(No.2013911131;2014KL002);教育部留学回国人员科研启动基金(No.J20120279)~~

摘　　要：用金标免疫层析读数仪和肉眼两种方法判读采用金标免疫层析试纸条检测的159份棘球蚴病患者血清和80份其他肝脏疾病患者血清。结果显示,金标免疫层析读数仪检测抗细粒棘球蚴抗体的敏感性(95.6%,152/159)、特异性(93.7%,75/80)和准确性(95.0%,227/239)均高于肉眼判读结果 (92.4%、85.0%和89.9%),而假阴性率(4.4%,7/159)和假阳性率(6.3%,5/80)均低于肉眼判读结果 (7.5%和15.0%)。One hundred and fifty-nine serum samples from hydatid disease patients and 80 serum samples from patients with other liver diseases were detected by gold-immunochromatographic assay, and read by naked eyes and the gold-immunochromatographic test strip reader. The sensitivity, specificity, and accuracy of eye-based method was 92.4% （147/159）, 85.0% （68/80）, and 89.9% （215/239）, which was lower than that of the reader detection （95.6%, 93.7%, 95.0%, respectively）. While, its false negative rate （7.5%, 12/159） and false positive rate （15.0%, 12/80） was higher than that of the reader detection （4.4% and 6.3%, respectively）.

关 键 词：金标免疫层析 定量测试 金标免疫层析读数仪 棘球蚴病 抗体 

分 类 号：R532.32[医药卫生—内科学]