灭螺微生物的选育及其效果观察  

Screening of Molluscacidal Microorganisms against Oncomelania hupensis and Their Effect

作　　者：崔国艳[1,2] 汪世平[1] 程红兵[2] 魏红[3] 何鑫[1] 闾丘思嘉 黄成铭[1] 

机构地区：[1]中南大学湘雅医学院寄生虫学教研室,长沙410078 [2]长治医学院微生物教研室,长治046000 [3]长治医学院附属和平医院感染管理科,长治046000

出　　处：《中国寄生虫学与寄生虫病杂志》2015年第3期234-236,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.81271862);国家科技支撑计划(No.2009BAI78B05)~~

摘　　要：从湖南省汨罗市血吸虫病防治研究实验基地的钉螺池中采集土壤样品15份,初筛出4株灭螺活性较强的菌株,分别为B8、B27、B36和B59。灭螺试验结果表明,与细菌发酵液组和菌体悬液组相比,4株菌株的发酵上清液组的灭螺效果最好。B59菌株的发酵上清液分别浸泡钉螺48和72 h后,钉螺的死亡率为73.3%(44/60)和96.7%(58/60),灭螺效果优于其他3株细菌。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)结果表明,B59菌株的上清液、发酵液和菌体悬液中均无蛋白条带出现。B59菌株为革兰氏阳性杆菌。分子系统进化分析表明B59菌株(GenBank登录号为KP146144)的ITS序列与蜡样芽孢杆菌相关序列(Gen Bank登录号为CP001746)的同源性达100%。Fifteen soil samples were collected from Oncomelania hupensis culture pond in Miluo Schistosomiasis Control and Prevention Base, Hunan Province. Four strains of bacteria were identified to have molluscacidal effects, numbered as B8, B27, B36 and B59. Compared with the fermentation broth groups and bacteria suspension groups, the fermentation supernatant groups of the four strains showed the strongest molluscacidal effect. The fermentation supernatant of B59 strain showed the best molluscacidal effect, with snail mortalit of 73.3% and 96.7% at 48 h and 72 h of treatment, respectively. SDS-PAGE revealed no proteins in fermentation supernatant, fermentation broth and bacteria suspension of B59 strain. Molecular phylogenetic analysis based on ITS sequence showed that the ITS sequence of strain B59 （accession No. KP146144） was 100% homologous to that of the same fragment of Bacillus cereus （accession No. CP001746）.

关 键 词：钉螺 微生物 选育 灭螺效果 分子系统发育 

分 类 号：R383.241[医药卫生—医学寄生虫学]