碱性亮氨酸拉链转录因子ATF样蛋白在狼疮小鼠肾脏组织中的表达及意义  

Expression of BATF, a member of the activator protein-1 family, in renal tissues of MRL/lpr mice

作　　者：彭宇声 黎倩[1] 王梦蕾[1] 曹灿[1] 赖宽[1] 曾抗[1] 

机构地区：[1]南方医科大学南方医院皮肤科,广东广州510515

出　　处：《南方医科大学学报》2015年第6期816-819,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81301371);广东省自然科学基金(2014A030313350);南方医科大学科研启动计划(B1012028)~~

摘　　要：目的探讨碱性亮氨酸拉链转录因子ATF样蛋白(basic leucine zipper transcription factor,ATF-like,BATF)在狼疮鼠肾脏组织中的表达及临床意义。方法以24周龄雌性MRL/lpr小鼠作为狼疮肾炎模型组,同龄C57BL/6雌性小鼠作为正常对照组。采用Western blot检测肾脏组织BATF的蛋白表达,采用RT-PCR检测肾脏组织中BATF、RORγt及IL-17 m RNA的表达水平,同时检测小鼠尿蛋白、血清抗ds-DNA抗体以及肾脏组织病理的表现,并对BATFm RNA及RORγt、IL-17 m RNA相关性进行分析。结果狼疮小鼠尿蛋白、血清抗ds-DNA抗体水平均高于正常小鼠(P<0.05)。狼疮小鼠肾脏组织病理主要表现为肾小球系膜细胞增生,肾间质大量炎细胞浸润,而正常小鼠无明显异常。狼疮小鼠肾脏组织BATF m RNA和蛋白的表达量均较正常小鼠升高(P<0.05)。狼疮小鼠肾脏组织RORγt、IL-17 m RNA表达量增多,且BATF m RNA的表达量与RORγt、IL-17 m RNA表达量呈正相关(r=0.945,0.876,P均<0.05)。结论在狼疮小鼠肾脏组织中BATF m RNA和蛋白的表达均高于对照组,BATF可能通过上调Thl7免疫反应参与狼疮性肾炎的发病。Objective To investigate the expression of BATF, a member of the activator protein- 1 family, in the renal tissues of mice with lupus nephritis. Methods The renal tissues from 24- week- old female MRL/lpr mice and age- and sex- matched C57BL/6 mice were examined for BATF protein expressions using Western blotting and for expressions of BATF, RORγt and IL-17 A m RNA using quantitative real- time PCR. The results of laboratory examinations were analyzed in relation with the histopathology of the mice. Results The urinary protein and ds-DNA levels were significantly higher in MRL/lpr mice than in the control mice（P〈0.05）. Compared to normal control mice, MRL/lpr mice showed obvious glomerular fibrosis and inflammatory cell infiltrating with significantly increased BATF protein and m RNA expressions（P〈0.05） and RORγt and IL-17 m RNA expressions in the renal tissues（P〈0.05）. In MRL/lpr mice, the expression of BATF m RNA was positively correlated with the RORγt and IL- 17 A m RNA expressions in the renal tissues. Conclusion The renal expressions of BATF protein and m RNA is increased in MRL/lpr mice. BATF may participate in the immunopathogenesis of lupus nephritis by enhancing Thl7 cell response.

关 键 词：BATF转录因子 系统性红斑狼疮 狼疮性肾炎 

分 类 号：R593.241[医药卫生—内科学]