麦粒灸“肝俞”对原发性肝癌癌前病变模型大鼠肝功能的影响  

Effects of moxibustion with seed-sized moxa cone at“Ganshu”(BL 18)on liver function in rats with precancerous lesion of hepatic cellular cancer

作　　者：柳杨[1] 侯中伟[1] 卢峻[1] 董峰[2] 王培[1] 贾文睿 王朝阳[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]中国中医科学院针灸医院

出　　处：《中国针灸》2015年第7期702-706,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金青年科学基金项目:81202760

摘　　要：目的:探讨麦粒灸"肝俞"对原发性肝癌(HCC)癌前病变期大鼠肝功能以及肝脏组织形态的影响。方法:将60只雄性Wistar大鼠随机分为正常组(10只)、模型组(20只)、疗程1组(15只)和疗程2组(15只)。采用腹腔注射二乙基亚硝胺(diethylnitrosamine,DEN)法制备HCC模型。正常组不干预;模型组只抓取按压;疗程1组、疗程2组自造模开始同时给予"肝俞"麦粒灸干预,隔日1次,分别持续1个疗程(20天)和2个疗程(40天)。各组大鼠分别于造模开始前1天、造模开始后第20天、40天和84天造模完成4个时间点取血,化学法进行血清丙氨酸氨基转移酶(alamine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate transaminase,AST)、γ-谷氨酰转肽酶(glutamyl transpeptidase,GGT)活性测定;并在造模结束时,将各组大鼠处死,观察大鼠肝脏组织形态学变化。结果:第1疗程结束后,疗程1组ALT、AST含量明显低于模型组(均P<0.05);第2疗程结束后,疗程2组ALT、AST、GGT含量较模型组低,但差异均无统计学意义(均P>0.05)。肝脏组织切片HE染色光镜下观察,模型组肿瘤灶诱导形成;疗程1组胞浆均匀,癌细胞数量少,仅见少量结节形成;疗程2组肝脏保持正常组织形态,细胞染色均匀,癌细胞数量少,无结节形成或仅见少量结节。结论:麦粒灸"肝俞"能够降低HCC模型大鼠血清ALT、AST、GGT含量,保护肝脏,并在一定程度上延缓DEN诱发大鼠HCC的癌前病变。Objective To explore the effects of moxibustion with seed-sized moxa cone at ＂Ganshu＂ （BL 18） on liver [unction and morphology in rat with precancerous lesion of hepatic cellular cancer （HCC）. Methods A total of 60 male Wistar rats were randomly divided into a normal group （10 rats）, a model group （20 rats）, a 20-day treatment group （15 rats） and a 40-day treatment group （15 rats）. HCC model was established by intraperitoneal injection of diethylnitrosamine （DEN）. Rats in the normal group received no treatment. Rats in the model group were treated with fixation. Rats in the 20-day treatment group and 40-day treatment group were treated by moxibustion with seed-sized moxa cone at ＂Ganshu＂ （BL 18）, once every other day, for 20 days and 40 days, respectively. Blood sample in each group was collected 1 d before model establishment, 20 d, 40 d and 84 d after model establishment. Chemical method was applied to test the activity of ALT （alamine aminotransferase）, AST （aspartare transaminase） and GGT （glutamyl transpeptidase）; at the end of model establishment, all the rats were sacrificed to observe the liver morphology changes. Results After the first therapeutic course, the content of ALT and AST in the 20-day treatment group was significantly lower than that in the model group （all P〈0.05） ; after the second therapeutic course, the content of ALT, AST and GGT in the 40-day treatment group was insignificantly lower than that in the model group （all P〉0.05）. Under light microscope, the slice of liver tissue indicated that primary tumor was induced in the model group, and the tumor cells were stained and irregular; the cytoplasm in the 20-day treatment group was even, and the tumor cells were few with several nodules alone. In the 40-day treatment group the liver morphology was normal and the staining was even; the tumor cells were few without nodules or a few. Conclusion Moxibustion with seed-sized moxa cone at ＂Ganshu＂ （BL 18） could reduce the ser

关 键 词：原发性肝癌 癌前病变 麦粒灸 穴 肝俞 肝功能 肝脏形态 

分 类 号：R246.1[医药卫生—针灸推拿学]