嗅鞘细胞分泌蛋白SC1促进神经元突触形成  

OECs secreted protein SC1 promotes neuronal synaptogenesis

作　　者：段答[1] 姜淼[2] 叶玉勤[1,3] 葛丽特[1,4] 贺晓生[3] 陈平[2] 卢明[1,4] 

机构地区：[1]解放军第163医院神经外科(湖南师范大学第二附属医院),湖南长沙410003 [2]湖南师范大学生命科学学院,湖南长沙410008 [3]第四军医大学西京医院神经外科,陕西西安710032 [4]湖南师范大学医学院,湖南长沙410006

出　　处：《中华神经外科疾病研究杂志》2015年第3期204-207,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(81371358);国家自然科学基金资助项目(81471264);湖南省自然科学基金资助项目(14JJ2060);湖南省教育厅资助科研项目(12C0238);湖南省教育厅研究生创新型项目(CX2014B237)

摘　　要：目的探讨嗅鞘细胞(OECs)诱导神经干细胞(NSCs)分化后,其分泌蛋白SC1对分化后神经元突触形成的作用。方法用小鼠OECs条件培养基诱导小鼠神经干细胞系C17.2分化后,用突触前膜抗体bassoon标记突触并计数,同时用Western blot检测bassoon的表达量。结果 OECs诱导组突触数目及bassoon的表达量明显高于全反式维甲酸(RA)诱导组,在RA诱导组加入小鼠SC1重组蛋白后,突触数目及bassoon的表达量与OECs诱导组接近。结论 OECs分泌蛋白SC1能促进C17.2 NSCs分化后神经元的突触形成。Objective The role of secreted protein SC1 in synaptogenesis after neural stem cells （ NSCs ） induced by olfactory ensheathing cells （OECs） is discussed.Methods Mouse NSCs line C17.2 was induced by mouse OECs conditioned medium, then the presynapses were labeled with primary antibody bassoon and the expression of bassoon was identified with Western blot.Results The synapse number and expression of bassoon in OECs group were significantly higher than those of RA group.After mouse SC1 recombinant protein added into retinoic acid （RA） group, the synapse number and the expression of bassoon were close to those of OECs group. Conclusion OECs secreted protein SC1 can promote synaptogenesis after C17.2 NSCs induced by OECs conditioned medium.

关 键 词：嗅鞘细胞 神经干细胞 SC1蛋白 突触形成 

分 类 号：R651.2[医药卫生—外科学]