铜藻岩藻黄质的提取工艺优化  

Study on Extraction of Fucoxanthin from Sargassum horneri

作　　者：胡永东[1] 吴柠 李易珍 闻正顺[1] 欧阳小琨[1] 杨立业[1] 徐银峰[1] 曲有乐[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江舟山316022

出　　处：《浙江海洋学院学报（自然科学版）》2014年第6期501-505,510,共6页Journal of Zhejiang Ocean University(Natural Science Edition)

基　　金：舟山市科技计划项目(2012C33004);浙江海洋学院2012年科研计划项目(面上项目)(X12M05);浙江海洋学院校级重大项目(X12ZD08);浙江省教育厅2013年度科研计划项目[年度科研计划项目(Y201328199)]

摘　　要：运用溶剂萃取法,并通过单因素试验、正交试验和响应曲面试验优化提取工艺的影响因素提取温度、液料比和提取时间,对铜藻中岩藻黄质的提取工艺进行研究。结果表明:最高提取率可为0.72 mg/g,相对应的提取条件为温度70℃,时间127 min,液料比68.3 m L/g。正交试验(0.71 mg/g)与响应曲面试验(0.72 mg/g)所得出的最佳条件接近。铜藻中含有一定量的岩藻黄质,试验优化的工艺稳定可行,可以为岩藻黄质的进一步开发提供一定的依据。To optimize the process of the organic solvent extraction of fucoxanthin from Sargassum horneri. Affecting factors for the extraction technique, such as extraction temperature, ratio of solvent to sample （volume/ weight）, and extraction time were optimized with the single factor test, orthogonal array design and response surface experiment. The result showed that： Maximum extraction rate of fucoxanthin was 0.72 mg/g when the extraction model is as follows： extraction temperature 70 ~C, the ratio of solvent to sample 68.3：1 （volume/weight, mL/ g）and extraction time 127 min. The optimum results between from the orthogonal array design and from response surface methodology were approximate. S. horneri is rich in fucoxanthin. The optimized extraction process in the study is stable and feasible to provide important reference meanings for further research and development of the fucoxanthin.

关 键 词：铜藻 岩藻黄质 工艺优化 

分 类 号：Q539[生物学—生物化学]