康脑液对脑缺血再灌注损伤大鼠血管内皮生长因子、脑源性神经生长因子、基质金属蛋白酶表达的影响  

Effect of Kangnaoye on Expression of VEGF, BDNF, MMP-9 after Cerebral Ischemia Reperfusion in Rats

作　　者：马建鹏[1] 常青[1] 刘亚丽[2] 薛茜[3] 邹玉安[3] 宋爱霞[3] 

机构地区：[1]河北北方学院 [2]河北北方学院附属第二医院心内科 [3]河北北方学院附属第一医院神经内科

出　　处：《神经药理学报》2014年第2期16-21,共6页Acta Neuropharmacologica

基　　金：河北省卫生厅课题基金资助项目(No.20090591)

摘　　要：目的:通过康脑液干预观察其对脑缺血再灌注损伤大鼠血管内皮生长因子(vascular endothelial growth factor,VEGF)、脑源性神经生长因子(brain derived neurotrophic factor,BDNF)、基质金属蛋白酶(matrix metalloproteinase-9,MMP-9)表达的影响,探讨康脑液对脑缺血再灌注损伤的保护机制。方法:将雄性SD大鼠随机分为假手术组、脑缺血再灌注模型组及康脑液28.6、14.3、7.15 g·kg-1·d-1剂量组(灌胃给药7 d),改进Longa等线栓法制备大鼠右侧大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)再灌注模型。于缺血2 h后再灌注(分别于再灌注后6 h、12 h、24 h、72 h、7 d处死大鼠);采用TTC染色法观察大鼠的脑梗死面积;免疫组织化学法观察大鼠脑组织VEGF、BDNF、MMP-9的表达。结果:比较各组缺血再灌注24 h大鼠脑梗死灶面积,发现28.6、14.3 g·kg-1·d-1剂量组较脑缺血再灌注模型组明显减小(P<0.05);与脑缺血再灌注模型组相比,28.6、14.3 g·kg-1·d-1剂量组各时间点的VEGF、BDNF表达量明显上调(P<0.01),MMP-9表达的阳性细胞明显减少(P<0.01),差异有统计学意义。结论:康脑液可促进大鼠局灶性脑缺血后脑组织中VEGF,BDNF的表达,同时抑制脑内MMP-9的表达,缩小脑梗死面积,发挥对神经血管单元(neurovascular unit,NVU)的保护作用,减轻脑缺血再灌注损伤。Objective：To observe the effect of Kangnaoye pretreatment on the expression of vascular endothelial growth factor （VEGF）, brain derived neurotrophic factor （BDNF）, matrix metalloproteinase-9 （MMP-9） in rats with cerebral ischemia-reperfusion injury,and further to explore the neurovacular unit protective mechanism of Kangnaoye against the injury of cerebral ischemia-reperfusion. Methods：Male Spraque-Dawley rats were randomly divided into：shamoperation group, ischemia-reperfusion group, Kangnaoye 28.6,14.3,7.15 g· kg -1. d-1 dose group （intragastric administration, 7 d）. Improved Zea Longa method was used to prepare cerebral ischemia-reperfusion model of rats. Reperfusion after ischemia for 2 h （rats were sacrificed respectively after reperfusion 6 h, 12 h,24 h,72 h,7 d）. TTC staining method was used for observing the infarct area of rat brain;Immunohistochemical method was used for observing the expression of VEGF, BDNF, MMP-9 in rats brain tissue. Results：The comparison of the infarct area between the groups 24 h after reperfusion ischemic found that the Kny High, Middle dose group significantly decreased the infarct area （P〈0.05）, along with the unregulated expression of VEGF, BDNF （P〈0.01）. The expression of MMP-9 was significantly decreased as compared to model group （P〈0.01）. Conclusion： Kangnaoye promoted the expression of VEGF, BDNF in brain tissue,inhibited the expression of MMP-9, decreased the infarct area and protected neurovascular unit against cerebral ischemia reperfusion injury.

关 键 词：康脑液 神经血管单元 血管内皮生长因子 脑源性神经生长因子 基质金属蛋白酶 

分 类 号：R965[医药卫生—药理学]