丹参水溶性有效成分对血管内皮细胞损伤保护作用的均匀设计-高通量筛选研究  

Application of Uniform Design-High Throughput Screening in Research of Vascular Endothelial Cells Protective Effect of Radix Salviae Miltiorrhizae Hydrosoluble Constituents

作　　者：田慧[1] 王倩[1] 梅艳飞 张丹参[2] 薛贵平[1] 

机构地区：[1]河北北方学院 [2]河北科技大学

出　　处：《神经药理学报》2014年第3期1-10,共10页Acta Neuropharmacologica

基　　金：国家自然科学基金项目(No.81274005)

摘　　要：目的：通过丹参七种水溶性有效成分多水平均匀设计配伍的血管内皮细胞保护作用筛选研究,评价中药有效成分的均匀设计-高通量筛选技术（uniform design-high throughput screening,UD-HTS）的应用.方法：选取原儿茶醛、原儿茶酸、咖啡酸、丹参素钠、迷迭香酸、丹酚酸A、丹酚酸B七种丹参水溶性单体有效成分七个水平（1×10-4～1×10-10 mol·L-1）进行均匀设计配伍组合,通过H2O2诱导的氧化应激损伤血管内皮细胞模型、血管内皮细胞营养剥夺模型评价各种有效成分配伍组合对药效的影响.结果：经过初筛和复筛,初步得到了2个最佳配伍组合样品（A2、B4）,其细胞存活率分别为（70±4）％,（76±3）％.不同的细胞损伤模型筛选出不同的最优配比.结论：均匀设计配伍与中药复方配伍有相似之处,均匀设计-高通量筛选技术是适用于传统多因素多水平组合特点的大规模药效筛选研究新方法.Objective：To evaluate the application of uniform design-high throughput screening technology in the study of the active ingredient of traditional Chinese medicine, vascular endothelial cells protective effect is detected by multifactor and multilevel and uniform design-high throughput screening （UD-HTS）method of Radix Salviae Miltiorrhizae hydrosoluble constituents. Methods： We performed uniform design compatibility study on seven Radix Salviac Miltiorrhizae hydrosoluble constitutcnts, each of which at seven concentration levels （1× 10^-4 1×10^10 mol· L^-1 reel. L-1） and their protective effect was assessed by the damage of vascular endothelial cells injured by H202 and serum deprivation. Results：After high-throughput screening and rescrvcning,the best Radix Salviac Miltiorrhizae hydrosoluble combination were obtained with A2 [ cell survival ratio was （70± 4） % ] and B4 [ cell survival ratio was （76±3 ） % ]. Differentcombination samples were identified with different vascular endothelial cells model which was damaged by H202 and serum deprivation. Conclusion ：Uniform design compatibility and the traditional Chinese medicine compound compatibility have similarities. Uniform design-high throughput screening technology is a reliable technology for massive potency screening of active ingredients of traditional Chinese medicine and compounds.

关 键 词：均匀设计-高通量筛选 丹参 水溶性成分 配比 

分 类 号：R965[医药卫生—药理学]