小鼠条件性电击社交恐惧行为模型的建立与评价  

Establishment and Evaluation of the Conditioned Foot-Shock as a Behaviour Model of Social Phobia in Mice

作　　者：赵楠[1] 张黎明[1] 李云峰[1] 

机构地区：[1]军事医学科学院毒物药物研究所

出　　处：《神经药理学报》2014年第4期15-19,共5页Acta Neuropharmacologica

摘　　要：目的：建立稳定而特异性强的小鼠社交恐惧行为模型。方法：采用针对社交接触行为给予条件性足底电击（O．7mA，1S，1～5次）的方式建立小鼠社交恐惧模型。分别于电击后d2和d15检测小鼠的社交探究行为，以探究时间百分比（investigationtime％，1T％）为社交恐惧行为评价指标。分别采用小鼠高架十宁迷宫实验、明暗穿箱实验和强迫性游泳实验评价小鼠的广泛性焦虑行为和抑郁样行为。结果：在条件性电击后d2和d15的社交探究实验中，与非丰十交刺激（non．socialstimulus，NSS）组比较，条件性社交刺激（conditionedsocialstimulus，CSS）组小鼠IT％均显著减少。在小鼠高架十字迷宫实验、明暗穿箱实验和强迫性游泳实验上，CSS组与NSS组各行为指标差异均无显著意义。结论：采用条件性足底电击方式可成功建立小鼠社交恐惧行为模型，模型症状可持续至少2周，且不伴随广泛性焦虑行为或抑郁样行为，具有良好的稳定性和特异性。Objective：To establish a stable and specific behaviour model of social phobia（SP） in mice. Methods：The conditioned foot-shock （0.7 mA, 1 s, 1-5 times） was used to induce a model of SP in mice. The social investigation behavior was detected on d 2 and d 15 after the foot-shock,with the percentage of investigation time （IT %） as a behavior index of SP. In addition, the elevated plus maze test （EPMT）, the light/dark transition test （L/DTT） and the forced swimming test （FST） were used to evaluate the generalized anxiety behavior or depression-like behavior of the model mice. Results：In the social investigation test, compared with the non-social stimulus（NSS）group,the IT % of conditioned social stimulus（CSS） group were significantly decreased on d 2 or d 15 day after the conditioned foot-shock. On the other hand,no significant differences were observed between NSS group and CSS group on the behavioral indexes of EPMT,L/DTT and FST. Conclusion：The SP model in mice was established successfully by the conditioned foot-shock paradigm. The model was stable and specific within at least 2 weeks and without the interference of generalized anxiety or depression- like behavior.

关 键 词：社交恐惧 行为模型 社交探究行为 ICR小鼠 

分 类 号：R965[医药卫生—药理学]