小鼠感染流感病毒后外周血IFN-α变化规律的研究  

Studies on the Regularity of IFN- alpha in Peripheral Blood of Mice Infected with Influenza Virus

作　　者：李艳[1] 宁秋[2] 韩德明[3] 姚海虹[3] 赵赫楠 赵丽辉[3] 

机构地区：[1]长春理工大学校医院,长春130022 [2]辽源职业技术学院医药分院,辽源136200 [3]长春理工大学生命科学技术学院,长春130022

出　　处：《长春理工大学学报（自然科学版）》2015年第3期156-159,共4页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省教育厅科研项目(201427)

摘　　要：流感病毒是引起呼吸道疾病的主要病原体,侵袭机体的上皮细胞和中性粒细胞产生具有生物活性的细胞因子。通过抽取感染了普通流感病毒病人的血液对小鼠进行滴鼻感染实验,通过ELISA实验检测小鼠血清中细胞因子IFN-α的含量及其变化情况,阐明普通流感病毒对小鼠机体防御系统免疫激活过程中IFN-α的变化规律。实验结果显示,产生I型干扰素(IFN-α)随着时间的增加而变化,逐渐趋于平稳,为下一步研制新型通用流感疫苗奠定基础。Influenza viruses are important pathogens causing respiratory disease, infecting organism epithelial cells and neutrophils producing bioactive cytokines. Mice were infected intranasally with the patient＇s blood by infecting the com-mon influenza virus,detecting content and its changes of serum cytokines IFN-αin mice by ELISA experiment,to clar-ify the common influenza viruses change of activation of IFN-alpha in the process of the body＇s defense system of im-munity in mice. Experimental results show that the production of type I interferon （IFN- alpha） varied with time in-crease,gradually stabilized,to lay a foundation for the further development of new universal flu vaccine.

关 键 词：流感病毒 细胞因子 诱导 IFN-Α 

分 类 号：R181.2[医药卫生—流行病学]