跑节省化标志的有氧能力相关尿游离miRNA表达谱特征  

Diagnostic Pattern of Urine Cell-Free microRNA Related to the Trainability of Aerobic Athletic Ability Indicated by Running Economy

作　　者：魏胜辉[1] 李岩[2] 

机构地区：[1]四川师范大学体育学院,四川成都610101 [2]鲁东大学体育学院,山东烟台264025

出　　处：《成都体育学院学报》2015年第4期111-117,共7页Journal of Chengdu Sport University

基　　金：国家自然基金项目(8173281);四川省教育厅项目(11SA055)

摘　　要：目的:miRNA参与调控运动性适应基本生理过程的基因选择性表达,其调控模式影响运动适应能力,导致运动能力及可训练性的个体差异。组织miRNA与尿游离miRNA(UCFmiRNA)在表达水平上密切相关,本研究分析UCF-miRNA表达谱特征与有氧运动能力可训练性水平的关系,构建关联模型用于运动员选材和训练。方法:k-means法对91名无训练背景的男性大学新生17周有氧训练前后跑节省化相对值(R-RE)变化率做聚类分析,将研究对象分为对训练的高敏感型组(HR组)和普通敏感型组(CR组)。提取和分析HR组UCF-miRNAs表达谱模式特征及其与有氧耐力指标的相关性。结果:12个在功能上与运动性适应调控相关的UCF-miRNAs构成的特异性表达差异谱与显著高于平均水平的R-RE提高率表型有关联,其表达水平变化率与有氧耐力相关指标变化率具有显著的相关性。结论:单次训练过程中UCFmiRNAs特征性表达谱模式与有氧运动能力的可训练性存在关联,可用来早期预测有氧运动能力发展潜力。Objective：miRNAs affect the trainability of athletic ability by regulating the essential processes of exercise adaption. The expression profile of Urine cell - free miRNAs （UCF - miRNAs）associates with the pattern of miRNA in tissue. The research is to build the model of UCF - miRNA expressing pattern reflecting the train-ability of aerobic athletic ability. Method：91 healthy male freshmen without any experience of aerobic training are engaged in a 17 - week aerobic training with the running economy （RE/ R - RE）before and after the train-ing were measured,and then the subjects are grouped as High Response group（HR group）and Common Re-sponse group（CR group）,depending on the result of the K - means clustering of growth ratio of R - RE. The characters of UCF - miRNAs’Differential Expression Profil were extracted and analyzed. Result：12 UCF -miRNAs are closely related to exercise adoption and their expressing patterns are extracted,validated and con-firmed to constitute the differential expression profile of HR group which is characteristic of higher growth ratio of R - RE with its Differential Expression Profile coincides with the positive characters of aerobic athletic abili-ty. Conclusion：UCF - miRNAs’Differential Expression Profile is closely related to the trainability of aerobic athletic ability,which can be used as a method to forecast the potential of aerobic athletic ability and to value the effect of training early and accurately.

关 键 词：跑节省化 有氧运动能力 尿游离miRNA 可训练性 运动员选材 

分 类 号：G804.2[文化科学—运动人体科学]