不同脂肪源饲粮育成猪肝脏转录组差异分析  

Differentially Analysis of Liver Transcriptomes of Finishing Pigs Fed Different Dietary Fats Sources

作　　者：李建平[1,2] 秦贵信[1] 赵志辉[3] 张巧灵[4] 王大力[3] 孙博兴[3] 姜海龙[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]河南牧业经济学院畜牧工程系,郑州450011 [3]吉林大学农业试验基地,长春130062 [4]吉林大学动物医学学院,长春130062

出　　处：《动物营养学报》2015年第7期2128-2139,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：吉林省安全优质肉猪现代生产关键技术示范(20140301013ny);国家科技支撑计划课题生猪健康养殖模式构建与示范(2012bad39b03)

摘　　要：本试验旨在肥育猪饲粮中分别添加红花籽油和椰子油,采集育成猪肝脏组织,进行高通量转录组测序,找出2种处理间的差异表达基因。利用Illumina HiSeqTM 2500高通量RNA-seq测序技术对2种处理育成猪肝脏进行转录组测序,使用TopHat2软件将测序得到的Reads序列与猪参考基因组(Sscrofa 10.2)序列比对,找出差异表达基因,并在Nr、GO和KEGG数据库中进行功能注释、富集分析和聚类分析。结果显示:红花籽油组和椰子油组肝脏差异表达基因共有938个,与椰子油组相比,红花籽油组表达上调基因有479个,下调基因有459个;GO功能分类注释到细胞组成、生物学过程和分子功能数据库中差异表达基因数分别有773、768和729个;注释到KEGG通路中差异表达基因数346个,显著富集通路为酮体生成与降解通路和类萜骨架生物合成通路(P<0.05)。The study was to detect differentially expressed genes in liver of finishing pigs which were divided into two groups and separately fed safflower seed oil and coconut oil. The chosen pigs were slaughtered and liver tissue were isolated and their mRNA were respectively extracted. The liver transcriptomes were analyzed by Illumina HiSeq TM 2500 high-throughput RNA sequencing system. All RNA-Seq reads were mapped on the reference pig genome （Sscrofa10.2） using TopHat2 software. All of differentially expressed genes were annotated using Nr, GO and KEGG databases. The results showed that there were 938 differentially expressed genes in liver between two groups. 479 genes were up-regulated and 459 genes were down-regulated in safflower seed oil group vs. coconut oil group. There were 773 differentially expressed genes annotated into database of cellular component, 768 into biological process, 729 into molecular function in GO databases. There were 346 differentially expressed genes annotated into KEGG databases, and clustered pathways of differentially expressed genes included synthesis and degradation of ketone bodies and terpenoid backbone biosynthesis （P 〈 0.05 ）.

关 键 词：红花籽油 椰子油 转录组 高通量RNA测序 差异表达基因 代谢通路 肝脏 育成猪 

分 类 号：S828[农业科学—畜牧学]