TRPV1通道介导的热生成是辛热(温)中药药性表征的重要模式  

TRPV1 channel-mediated thermogenesis is a common mode for the Chinese pungent-hot or pungent-warm herbs to demonstrate their natures

作　　者：隋峰[1] 戴丽[1] 李倩[1] 周海玉[1] 詹红丹[1] 霍海如[1] 姜廷良[1] 

机构地区：[1]中国中医科学院中药研究所,北京100070

出　　处：《药学学报》2015年第7期836-841,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81274112;81373986;81473372);北京市自然科学基金资助项目(7152106);中国中医科学院传承项目(CM2014GD3002);中国中医科学院中药研究所自主选题项目(ZZ2014056;ZZ2014027)

摘　　要：本文探索了辛热(温)性中药的现代科学内涵和分子机制。通过背根神经节(DRG)神经元的原代培养和瞬变感受器电位离子通道蛋白香草酸亚型1(TRPV1)基因转染HEK293细胞的方式获取了TRPV1通道原位和异源表达体系。在此基础上,采用共聚焦显微成像法对辣椒中的主要活性成分—辣椒素干预调节TRPV1通道的作用进行了研究;同时基于小鼠棕色脂肪组织(BAT)、直肠和皮肤温度搭建了三位一体的热电偶检测技术和方法,检测和评价了辣椒素对小鼠机体能量代谢的影响。两种表达体系中的TRPV1通道均能被辣椒素激活,且能够被TRPV1通道的特异性抑制剂—辣椒平(capsazepine)所拮抗;同时发现辣椒素给药后,机体的核心温度(core body temperature)和BAT温度先下降、后升高,但以升高为主;尾部皮肤温度给药后短暂升高后下降至基线。结果表明,通过激活TRPV1通道,进而提高机体热能的产生、实现温中散寒的生物效应,可能是辣椒等辛热(温)中药表征药性的一种重要分子机制和科学基础。To further uncover the scientific significance and molecular mechanism of the Chinese herbs with pungent hot or warm natures, endogenous and exogenous expression systems were established by isolation of dorsal root ganglion（DRG） neurons and transfection of HEK293 cells with TRPV1 channel gene separately. On this basis, the regulation action of capsaicin, one main ingredient from chili pepper, on TRPV1 channel was further explored by using confocal microscope. Besides, the three-sites one-unit technique and method were constructed based on the brown adipose tissue（BAT）, anal and tail skin temperatures. Then the effect of capsaicin on mouse energy metabolism was evaluated. Both endogenous and exogenous TRPV1 channel could be activated and this action could be specifically blocked by the TRPV1 channel inhibitor capsazepine. Simultaneously, the mice＇s core body temperature and BAT temperature fall down and then go up, accompanied by the increase of temperature of the mice＇s tail skin. Promotion of the energy metabolism by activation of TRPV1 channel might be the common way for the pungent-hot（warm） herbs to demonstrate their natures.

关 键 词：TRPV1通道 辣椒 辛热中药 中药药性 棕色脂肪组织 

分 类 号：R285[医药卫生—中药学]