小承气汤、厚朴三物汤及厚朴大黄汤中蒽醌类、木脂素类和黄酮类成分含量测定  

Simultaneous determination and quantitation of anthraquinones, lignans and flavonoids in Xiao-Cheng-Qi Tang, Hou-Po-San-Wu Tang and Hou-Po-Da-Huang Tang by HPLC

作　　者：陈立[1] 李涛[2] 王彦礼[1] 董宇[3] 庄帅星 王敦方 杨伟鹏[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院医学实验中心,北京100700 [3]广安门医院,北京100053

出　　处：《药学学报》2015年第7期887-892,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81273662,30801542);中国中医科学院自主业务选题项目(ZZ2014061,ZZ2014020)

摘　　要：建立同时定量小承气汤类方中蒽醌类、木脂素类和黄酮类成分的HPLC分析方法,并应用于小承气汤、厚朴三物汤、厚朴大黄汤的含量测定。用Agilent Zorbax SB-C18（4.6 mm×250 mm,5μm）色谱柱;0.5%乙酸和乙腈为流动相,梯度洗脱;流速1.0 m L·min-1;柱温30℃;进样量10μL;254 nm和294 nm波长同时检测。结果建立了没食子酸、肉桂酸、番泻苷A、番泻苷B、大黄酸、大黄素、芦荟大黄素、大黄酚、大黄素甲醚、厚朴酚、和厚朴酚、芸香柚皮苷、柚皮苷、橙皮苷、新陈皮苷、橙皮素、柚皮素和川陈皮素等18个成分的线性回归方程,方法的回收率为94.28%~99.89%,精密度的RSD值在5%以内,样品16 h内稳定。小承气汤、厚朴三物汤、厚朴大黄汤中蒽醌类、木脂素类和黄酮类成分含量有一定差别,小承气汤中蒽醌类和黄酮类成分含量最多,厚朴三物汤中木脂素类成分最多。本方法专属性、准确度、精密度和重复性良好,可用于小承气汤类方中蒽醌类、木脂素类和黄酮类成分含量测定,也可为其质量控制提供方法学支持。A simple and selective HPLC method for simultaneous determination and quantification of anthraquinones, lignans and flavonoids in Xiao-Cheng-Qi Tang（XCQT）, Hou-Po-San-Wu Tang（HPSWT） and Hou-Po-Da-Huang Tang（HPDHT） was developed and validated. An Agilent Zorbax SB-C18（4.6 mm × 250 mm, 5 μm） column with the mobile phase of acetonitrile and 0.5% acetic acid aqueous solution in gradient elution mode was used. The flow rate was 1.0 m L·min-1 at 30 ℃, and injection volume was 10 μL. The detection wavelength was set at 254 nm and 294 nm simultaneously for the quantitative analysis. The current HPLC assay was validated for linearity, intra-day and inter-day precisions, accuracy, recovery and stability. The method was applied to the content comparison of the gallic acid, cinnamic acid, sennoside A, sennoside B, rhein, emodin, aloe-emodin, chrysophanol, physcion, magnolol, honokiol, narirutin, naringin, hesperidin, neohesperidin, hesperetin, naringenin and nobiletin in XCQT, HPSWT and HPDHT. The good linear equations of eighteen constituents were obtained within the investigated ranges（r 0.998）. The recovery of the method was 94.28%-99.89% and the precision was less than 5%. The sample was stable within 16 h. There were some differences between the contents of anthraquinones, lignans and flavonoids in analogous formulae about XCQT. XCQT contained the greatest abundance of anthraquinones and flavonoid, HPSWT contained the greatest abundance lignans. In conclusion, the methods are simple, low-cost, precise, accurate and reliable for the determination of eighteen constituents in analogous formulae about XCQT, and these results provide methodological support for its quality control.

关 键 词：小承气汤 高效液相色谱法 蒽醌 木脂素 黄酮 

分 类 号：R917[医药卫生—药物分析学]