沙眼衣原体感染血清学筛查候选蛋白组合的优化  

Optimization of immunodominant protein combinations for serological screening for Cldamydia trachomatisinfection

作　　者：高西波 肖萌[1] 张新美[1] 马璟玥[1] 王敬[1] 刘全忠[1] 齐蔓莉[1] 

机构地区：[1]天津医科大学总医院皮肤性病科,300052

出　　处：《中华皮肤科杂志》2015年第7期463-466,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81201355）

摘　　要：目的优化适用于沙眼衣原体感染血清学筛查的候选抗原蛋白组合。方法收集天津医科大学总医院性病门诊经胶体金法检测沙眼衣原体感染者的血清和泌尿生殖道分泌物拭子各50份，胶体金法检测沙眼衣原体未感染者的血清和泌尿生殖道分泌物拭子各30份，此30份血清微量免疫荧光（MIF）法检测为阴性。以沙眼衣原体蛋白Hsp60和MOMP为参照，选择沙眼衣原体Pgp3、CPAF、CT143、CT101、CT694、CT875、CT813、Inca共8种免疫优势蛋白为抗原，检测血清中的相应抗体，将该结果与血清MIF检测和泌尿生殖道分泌物沙眼衣原体细胞培养两种传统金标准作比较，确定具有最高敏感性和特异性的抗原蛋白组合。结果50份胶体金法阳性标本中，MIF检测阳性44份，阴性6份；细胞培养阳性14份，阴性36份。沙眼衣原体Pgp3、CT694和CT875这3种免疫优势蛋白组合的血清学实验结果与MIF阳性符合率达到97．73％（43／44）。30份胶体金法检测沙眼衣原体阴性标本中，30份血清标本经MIF检测全为阴性，也未检出上述3种蛋白抗体。结论Pgp3、CT694与CT875免疫优势蛋白组合用于沙眼衣原体感染的血清学初筛具有很好的敏感性和特异性，且操作简单，易于推广。Objective To optimize immunodominant protein combinations for serological screening for Chlaraydia trachomatis （Ct） infection. Methods Both serum and genital swab samples were collected from 50 patients with Ct infection confirmed by colloidal gold immunochromatographic assay （GICA）, and 30 GICA-negative clients without Ct infection at a sexually transmitted disease （STD） clinic in Tianjin Medical University General Hospital. The 30 serum samples from GICA-negative clients were also negative for microimmunofluoreseence （MIF） assay. Eight Ct immunodominant proteins, including Pgp3, CPAF, CT143, CTIO1, CT694, CT875, CT813 and IncA, were selected as antigens to detect corresponding antibodies in the serum samples by enzyme-linked immunosorbent assay （ELISA） with the Ct proteins Hsp60 and major outer membrane protein （MOMP） as references. The results of ELISA were compared with those of the traditional gold standard method MIF assay to determine the immunodominant protein combination with the highest sensitivity and specificity. Results Of the 50 serum samples from patients with Ct infection, 44 were positive and 6 negative by MIF. The results of ELISA with the combination of immunodominant proteins Pgp3, CT694 and CT875 as antigens were 97.73% （43/44） consistent to those of MIF assay. Of the 30 serum samples from GICA-negative clients, all were negative by MIF. Meanwhile, no antibody was detected against any of the immunodominant proteins Pgp3, CT694 and CT875 in any of the serum samples from GICA-negative clients. Conclusions The ELISA with the combination of immunodominant proteins Pgp3, CT694 and CT875 as antigens has good sensitivity and specificity for serological screening for Ct infection, and is simple to operate and easy to popularize.

关 键 词：沙眼衣原体 免疫显性表位 血清学试验 荧光免疫测定 

分 类 号：R759[医药卫生—皮肤病学与性病学]