中国特有金钱槭属植物物种分化研究  

Interspecific Differentiation of the Endemic Plant Genus Dipteronia Oliv.

作　　者：柏国清[1,2,3] 李思锋[1,2] 李为民[1,2] 李忠虎[3] 杨娟[4] 赵桂仿[3] 

机构地区：[1]陕西省西安植物园 [2]陕西省植物研究所,西安710061 [3]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,西安710069 [4]西安交通大学环境与疾病相关基因教育部重点实验室生物医学研究实验中心/医学院遗传学与分子生物学系,西安710061

出　　处：《西北植物学报》2015年第6期1123-1128,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31470311);教育部博士点基金优先发展领域(20136101130001);陕西省财政厅项目(2010JM5005);陕西省科学院科技计划(2011-K03)

摘　　要：金钱槭属(Dipteronia Oliv.)隶属于槭树科(Aceraceae),是北温带植物区系中古老、残遗的木本属之一,为中国特有属,属下仅包括金钱槭(D.sinensis Oliv.)和云南金钱槭(D.dyerana Henry)2个物种。本实验从形态学、分子遗传学及基因序列分析等方面对金钱槭(D.sinensis Oliv.)和云南金钱槭(D.dyerana Henry)进行对比研究,探讨其物种间分化程度。结果表明:(1)形态学研究显示两物种形态性状存在显著差异,表明其在形态上发生了显著分化;(2)基因组DNA的AFLP标记分析表明,两物种间存在极显著遗传分化(ΦST=0.390 4),在物种水平及群体水平,两物种遗传多样性及遗传变异水平皆有明显差异(金钱槭>云南金钱槭);(3)从GenBank数据库中选出2物种已知的叶绿体基因(psbM-trnD、trnD-trnT、trnL、trnL-trnF、rpl16)和核基因ITS(ITS1、5.8SRNA、ITS2)进行序列进化比对分析,t-test检验表明,2物种叶绿体基因序列间有极显著差异。研究结果表明:金钱槭和云南金钱槭两物种间产生了极显著的分化,推测可能由于长期的地理隔离所致。The genus Dipteronia Oliv.（Aceraceae） is endemic in China which consists of two species：D.sinensis Oliv.and D.dyerana Henry.Dipteronia is one of the woody relic genera in the floristic regions of the northern temperate zone.In this study,we carried out a comparative study of D.sinensis Oliv.and D.dyerana Henry.to explore their degree of differentiation between two species from the aspects of morphology,molecular genetics and gene sequence analysis.（1）Morphological studies indicated that the presence of two species in morphological characters have significant differences.（2）Molecular genetic studies based on the AFLP of genomic DNA markers were also used to analyze the two species.And whether they are in species level or group level,the results showed that there was extremely significant genetic differentiation between the two species（ΦST=0.390 4）.The level of genetic variation and genetic diversity of the two species have distinct differences（D.sinensis Oliv.〉D.dyerana Henry）.（3）We also downloaded several chloroplast genes （psbM-trnD,trnD-trnT,trnL,trnL-trnF,rpl16） and nuclear gene sequences of ITS （ITS1,5.8SRNA,ITS2） of two known species from GenBank to analyze and compare their differentiation.t-test showed a significant difference between the two species occurred in chloroplast gene sequences.Hence,the results of the present study showed that the D.sinensis Oliv.and D.dyerana Henry have a extremely significant differentiation.And we infer the main reasons of the significant differentiation between the two species attributed to the long-term geographic isolation between them.

关 键 词：金钱槭 云南金钱槭 形态学 分子遗传学 种间分化 

分 类 号：Q789[生物学—分子生物学] Q941.3